Delineation of several DR-restricted tetanus toxin T cell epitopes

Détails

ID Serval
serval:BIB_1B85D0E6F531
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Delineation of several DR-restricted tetanus toxin T cell epitopes
Périodique
Journal of Immunology
Auteur(s)
Demotz  S., Lanzavecchia  A., Eisel  U., Niemann  H., Widmann  C., Corradin  G.
ISSN
0022-1767 (Print)
Statut éditorial
Publié
Date de publication
01/1989
Volume
142
Numéro
2
Pages
394-402
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jan 15
Résumé
We have characterized five human T cell clones specific for tetanus toxin. The combination of different techniques allowed us to precisely map two T cell epitopes within fragments 830-843 and 1273-1284 of tetanus toxin, as formally demonstrated by the use of corresponding synthetic peptides. The three other T cell clones were specific for regions 2-602, 604-742, and 865-1315 of tetanus toxin, respectively. The five T cell clones were shown to be restricted to HLA-DR Ag. Furthermore, the allele of HLA-DR utilized by the various epitopes has been determined. The use of HLA-DR-transfected L cells as APC directly demonstrated that two epitopes, one of which represented by fragment 1273-1284, were recognized in association with HLA-DRw52a. For the other three T cell epitopes, the data strongly suggested they were recognized in association with HLA-DR5. Finally, a sixth T cell clone was shown to be specific for tetanus toxoid, the vaccinal preparation of tetanus toxin, and not for other tetanus toxin fragments. This indicated that immunization with tetanus toxoid probably elicits a T cell response directed only in part against native tetanus toxin.
Mots-clé
Alleles Amino Acid Sequence B-Lymphocytes/immunology Cell Line Clone Cells/immunology Epitopes/*immunology HLA-DR Antigens/genetics/*immunology Humans Hydrolysis Lymphocyte Activation Molecular Sequence Data Peptide Fragments/immunology/isolation & purification Peptide Hydrolases Peptide Mapping Recombinant Proteins/immunology/isolation & purification/pharmacology T-Lymphocytes/*immunology Tetanus Toxin/*immunology/isolation & purification/pharmacology
Pubmed
Web of science
Création de la notice
24/01/2008 14:43
Dernière modification de la notice
20/08/2019 12:52
Données d'usage