Subcellular localization of Type VI secretion system assembly in response to cell-cell contact.
Détails
ID Serval
serval:BIB_1B5B68C78741
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Subcellular localization of Type VI secretion system assembly in response to cell-cell contact.
Périodique
The EMBO journal
ISSN
1460-2075 (Electronic)
ISSN-L
0261-4189
Statut éditorial
Publié
Date de publication
04/07/2022
Peer-reviewed
Oui
Volume
41
Numéro
13
Pages
e108595
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Résumé
Bacteria require a number of systems, including the type VI secretion system (T6SS), for interbacterial competition and pathogenesis. The T6SS is a large nanomachine that can deliver toxins directly across membranes of proximal target cells. Since major reassembly of T6SS is necessary after each secretion event, accurate timing and localization of T6SS assembly can lower the cost of protein translocation. Although critically important, mechanisms underlying spatiotemporal regulation of T6SS assembly remain poorly understood. Here, we used super-resolution live-cell imaging to show that while Acinetobacter and Burkholderia thailandensis can assemble T6SS at any site, a significant subset of T6SS assemblies localizes precisely to the site of contact between neighboring bacteria. We identified a class of diverse, previously uncharacterized, periplasmic proteins required for this dynamic localization of T6SS to cell-cell contact (TslA). This precise localization is also dependent on the outer membrane porin OmpA. Our analysis links transmembrane communication to accurate timing and localization of T6SS assembly as well as uncovers a pathway allowing bacterial cells to respond to cell-cell contact during interbacterial competition.
Mots-clé
Bacterial Proteins/genetics, Bacterial Proteins/metabolism, Protein Transport, Type VI Secretion Systems/genetics, Type VI Secretion Systems/metabolism, cell-cell contact, cell-surface contact, dynamic localization, structured illumination microscopy, type VI secretion
Pubmed
Web of science
Open Access
Oui
Création de la notice
22/07/2024 16:10
Dernière modification de la notice
27/07/2024 7:01