Improved fertilization and implantation rates after non-touch zona pellucida microdrilling of mouse oocytes with a 1.48 microm diode laser beam

Détails

ID Serval
serval:BIB_1B5AE138F320
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Improved fertilization and implantation rates after non-touch zona pellucida microdrilling of mouse oocytes with a 1.48 microm diode laser beam
Périodique
Human Reproduction
Auteur(s)
Germond  M., Nocera  D., Senn  A., Rink  K., Delacretaz  G., Pedrazzini  T., Hornung  J. P.
ISSN
0268-1161 (Print)
Statut éditorial
Publié
Date de publication
05/1996
Volume
11
Numéro
5
Pages
1043-8
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May
Résumé
The safety of microdrilling the zona pellucida of mouse oocytes with a 1.48 microm diode laser has been investigated by determining the ability of mouse oocytes to fertilize in vitro and develop in vivo. Mice born after transfer of control and zona pellucida-microdrilled embryos into foster mothers were submitted to anatomical and immunohistochemical investigations, and their aptitude to breed was assessed in two subsequent generations. Decoronization of the oocytes with hyaluronidase induced a reduction of the fertilization and implantation rates, which was attributed to a zona hardening phenomenon. After laser zona pellucida microdrilling, these rates were restored to those obtained with embryos derived from untreated oocyte-cumulus complexes. Pups derived from zona pellucida microdrilled embryos were comparable with those obtained from control embryos, confirming the lack of deleterious effects of the laser treatment. In conclusion, the 1.48 microm diode laser allows safe microdrilling of the zona pellucida of mouse oocytes after decoronization with hyaluronidase. Based on the health of the F2 generation and the lack of neuroanatomical and neurochemical differences, we concluded that this technology may be investigated in the human, particularly when the zona pellucida represents the main impediment for fertilization or embryo hatching.
Mots-clé
Animals Brain/anatomy & histology Embryo Transfer Female Fertilization in Vitro/*methods Immunohistochemistry *Lasers Male Mice *Microsurgery Pregnancy Reproduction Sperm Count Spinal Cord/anatomy & histology *Zona Pellucida
Pubmed
Web of science
Création de la notice
24/01/2008 14:22
Dernière modification de la notice
20/08/2019 12:52
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