Optimum in vitro expansion of human antigen-specific CD8 T cells for adoptive transfer therapy.
Détails
ID Serval
serval:BIB_1AC9A366B9C2
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Optimum in vitro expansion of human antigen-specific CD8 T cells for adoptive transfer therapy.
Périodique
Clinical and experimental immunology
ISSN
0009-9104
Statut éditorial
Publié
Date de publication
2005
Peer-reviewed
Oui
Volume
142
Numéro
2
Pages
292-302
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Résumé
Increasing evidence suggests that adoptive transfer of antigen-specific CD8(+) T cells could represent an effective strategy in the fight against chronic viral infections and malignancies such as melanoma. None the less, a major limitation in the implementation of such therapy resides in the difficulties associated with achieving rapid and efficient expansion of functional T cells in culture necessary to obtain the large numbers required for intravenous infusion. Recently, the critical role of the cytokines interleukin (IL)-2, IL-7 and IL-15 in driving T cell proliferation has been emphasized, thus suggesting their use in the optimization of expansion protocols. We have used major histocompatibility complex (MHC) class I/peptide multimers to monitor the expansion of antigen-specific CD8 T lymphocytes from whole blood, exploring the effect of antigenic peptide dose, IL-2, IL-7 and IL-15 concentrations on the magnitude and functional characteristics of the antigen-specific CD8(+) T cells generated. We show here that significant expansions of antigen-specific T cells, up to 50% of the CD8(+) T cell population, can be obtained after a single round of antigen/cytokine (IL-2 or IL-15) stimulation, and that these cells display good cytolytic and interferon (IFN)-gamma secretion capabilities. Our results provide an important basis for the rapid in vitro expansion of autologous T cells from the circulating lymphocyte pool using a simple procedure, which is necessary for the development of adoptive transfer therapies.
Mots-clé
Adoptive Transfer, Antigens, Neoplasm, CD8-Positive T-Lymphocytes, Cell Culture Techniques, Cell Division, Cell Line, Dose-Response Relationship, Immunologic, Epitopes, T-Lymphocyte, Humans, Immunophenotyping, Interleukin-15, Interleukin-2, Interleukin-7, Melanoma, Neoplasm Proteins
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/01/2008 12:13
Dernière modification de la notice
20/08/2019 13:51