Malaria parasite chitinase and penetration of the mosquito peritrophic membrane

Détails

ID Serval
serval:BIB_1AA29E3E4E49
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Malaria parasite chitinase and penetration of the mosquito peritrophic membrane
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur(s)
Huber  M., Cabib  E., Miller  L. H.
ISSN
0027-8424 (Print)
Statut éditorial
Publié
Date de publication
04/1991
Volume
88
Numéro
7
Pages
2807-10
Notes
Journal Article --- Old month value: Apr 1
Résumé
Malaria parasites (ookinetes) appear to digest the peritrophic membrane in the mosquito midgut during penetration. Previous studies demonstrated that lectins specific for N-acetylglucosamine bind to the peritrophic membrane and proposed that the membrane contains chitin [Rudin, W. & Hecker, H. (1989) Parasitol. Res. 75, 268-279]. In the present study, we show that the peritrophic membrane is digested by Serratia marcescens chitinase (EC 3.2.1.14), leading to the release of N-acetylglucosamine and fragmentation of the membrane. We also report the presence of a malaria parasite chitinase that digests 4-methylumbelliferyl chitotriose. The enzyme is not detectable until 15 hr after zygote formation, the time required for maturation of the parasite from a zygote to an ookinete, the invasive form of the parasite. At 20 hr, the enzyme begins to appear in the culture supernatant. The chitinase extracted from the parasite and found in the culture supernatant consists of a major band and two minor bands of activity on native polyacrylamide gel electrophoresis. The presence of chitin in the peritrophic membrane, the disruption of the peritrophic membrane during invasion, and the presence of chitinase in ookinetes suggest that the chitinase in ookinetes is used in the penetration of the peritrophic membrane.
Mots-clé
Aedes/*physiology Animals Cell Membrane/physiology Chickens Chitin/analysis Chitinase/isolation & purification/*metabolism Fertilization *Host-Parasite Relations Leukocytes/*parasitology Plasmodium gallinaceum/enzymology/*physiology Substrate Specificity
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 16:39
Dernière modification de la notice
20/08/2019 12:51
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