Tamoxifen loaded folic acid armed PEGylated magnetic nanoparticles for targeted imaging and therapy of cancer.
Détails
ID Serval
serval:BIB_1A76D902AAC3
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Tamoxifen loaded folic acid armed PEGylated magnetic nanoparticles for targeted imaging and therapy of cancer.
Périodique
Colloids and Surfaces. B, Biointerfaces
ISSN
1873-4367 (Electronic)
ISSN-L
0927-7765
Statut éditorial
Publié
Date de publication
2013
Volume
106
Pages
117-125
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Résumé
Magnetic nanoparticles (MNPs) have been widely used as drug delivery nanosystems and contrast agent for imaging and detection. To engineer multifunctional nanomedicines for simultaneous imaging and therapy of cancer cells, in the current study, we synthesized tamoxifen (TMX) loaded folic acid (FA) armed MNPs to target the folate receptor (FR) positive cancer cells. To this end, Fe3O4 nanoparticles (NPs) were synthesized through thermal decomposition of Fe(acac)3. Polyethylene glycol (PEG) was treated with excess bromoacetyl chloride (BrAc) and then with 3-aminopropyltriethoxysilane (APS) to synthesize bromoacetyl-terminal polyethylene glycol silane (APS-PEG-BrAc). The latter complex was treated with protected ethylene diamine to form a bifunctional PEG compound containing triethoxysilane at one end and amino group at the other end (APS-PEG-NH2). The Fe3O4-APS-PEG-NH2 NPs were prepared through self-assembly of APS-PEG-NH2 on MNPs, while the amino groups at the end of Fe3O4-APS-PEG-NH2 were conjugated with folic acid (FA), then loaded with TMX (Fe3O4-APS-PEG-FA-TMX). The average size of "Fe3O4-APS-PEG-FA-TMX" NPs was approximately 40 nm. The engineered MNPs were further characterized and examined in the human breast cancer MCF-7 cells that express FR. The TMX loaded MNPs (with loading efficiency of 49.1%) showed sustained liberation of TMX molecules (with 90% release in 72 h). Fluorescence microcopy and flow cytometry analyses revealed substantial interaction of Fe3O4-APS-PEG-FA-TMX NPs with the FR-positive MCF-7 cells. Cytotoxicity analysis resulted in significant growth inhibition in MCF-7 cells treated with Fe3O4-APS-PEG-FA-TMX NPs. Based on these findings, the TMX-loaded FA-armed PEGylated MNPs as a novel multifunctional nanomedicine/theranostic for concurrent targeting, imaging and therapy of the FR-positive cancer cells.
Mots-clé
Antineoplastic Agents, Phytogenic/administration & dosage, Antineoplastic Agents, Phytogenic/therapeutic use, Breast Neoplasms/pathology, Cell Line, Tumor, Drug Screening Assays, Antitumor, Female, Flow Cytometry, Folic Acid/administration & dosage, Folic Acid/therapeutic use, Humans, Microscopy, Electron, Scanning, Microscopy, Electron, Transmission, Microscopy, Fluorescence, Polyethylene Glycols/chemistry, Tamoxifen/administration & dosage, Tamoxifen/therapeutic use
Pubmed
Web of science
Création de la notice
14/10/2014 12:43
Dernière modification de la notice
20/08/2019 13:51