PCR en microbiologie: de l'amplification de l'ADN a l'interpretation du résultat [PCR in microbiology: from DNA amplification to results interpretation].

Détails

ID Serval
serval:BIB_19E3E7FCEB18
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Institution
Titre
PCR en microbiologie: de l'amplification de l'ADN a l'interpretation du résultat [PCR in microbiology: from DNA amplification to results interpretation].
Périodique
Revue Médicale Suisse
Auteur(s)
Jaton K., Greub G.
ISSN
1660-9379 (Print)
ISSN-L
1660-9379
Statut éditorial
Publié
Date de publication
04/2007
Volume
3
Numéro
106
Pages
931-938
Langue
français
Notes
Publication types: English Abstract ; Journal Article ; Review
Publication Status: ppublish
Résumé
Nucleic acid amplification (PCR) allows a relatively rapid and accurate diagnosis of infection caused by intracellular bacteria and other fastidious pathogens for which culture is difficult or impossible. Thus, Chalamydia trachomatis PCR largely improved our ability to diagnose chlamydial infection. PCR also exhibits a high sensitivity and a high reproductibility. However PCR has limitations that have to be taken into consideration to appropriately interpret the results. Such interpretation is difficult and should take into account the characteristics of the test, the microbial agent identified, the sample tested, and the clinical presentation. It is therefore essential that clinical microbiologists and clinicians share their knowledge and expertise to interpret PCR results at both clinical and analytical levels.
Mots-clé
Algorithms, DNA, Bacterial/isolation & purification, Humans, Polymerase Chain Reaction
Pubmed
Création de la notice
25/01/2008 15:27
Dernière modification de la notice
20/08/2019 13:51
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