Dimeric tRNA precursors in yeast

Détails

ID Serval
serval:BIB_19D7FF10225B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Dimeric tRNA precursors in yeast
Périodique
Nature
Auteur(s)
Schmidt  O., Mao  J., Ogden  R., Beckmann  J., Sakano  H., Abelson  J., Soll  D.
ISSN
0028-0836 (Print)
Statut éditorial
Publié
Date de publication
10/1980
Volume
287
Numéro
5784
Pages
750-2
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct 23
Résumé
Two DNA fragments, each containing tRNA(Arg)3 and a tRNA(Asp) gene in close conjunction, have been isolated from different genomic regions of Saccharomyces cerevisiae. Nucleotide Nucleotide sequence analysis of the gene regions revealed that in both fragments the tRNA(Arg)3 coding region is located 5'-proximal to the tRNA(Asp) coding region. They are separated by an identical spacer of 10 nucleotides. Although the 5'-flanking sequences are different in the two plasmids, some similarities are observed. To test the mode of expression of this gene configuration, we transcribed the DNA fragments in a Xenopus oocyte nuclear extract. Specific transcription of the yeast tRNA genes took place in an RNA precursor which comprised both tRNA species. We report here that the precursor RNA was processed to the mature-sized tRNA molecules, indicating the presence of an enzyme activity in the Xenopus nucleus capable of cutting a dimeric tRNA precursor. This is the first observation of a eukaryotic dimeric tRNA precursor.
Mots-clé
Animals Base Sequence Endonucleases/metabolism Genes Nucleic Acid Precursors/*metabolism RNA, Transfer/*biosynthesis/genetics Saccharomyces cerevisiae/*genetics Transcription, Genetic Xenopus laevis/genetics
Pubmed
Web of science
Création de la notice
25/01/2008 17:18
Dernière modification de la notice
20/08/2019 13:50
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