Nerve growth factor- and neurotrophin-3-releasing guidance channels promote regeneration of the transected rat dorsal root

Détails

ID Serval
serval:BIB_19B831C23BCC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Nerve growth factor- and neurotrophin-3-releasing guidance channels promote regeneration of the transected rat dorsal root
Périodique
Experimental Neurology
Auteur(s)
Bloch  J., Fine  E. G., Bouche  N., Zurn  A. D., Aebischer  P.
ISSN
0014-4886
Statut éditorial
Publié
Date de publication
12/2001
Peer-reviewed
Oui
Volume
172
Numéro
2
Pages
425-32
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec
Résumé
Dorsal roots have a limited regeneration capacity after transection. To improve nerve regeneration, the growth-promoting effects of the neurotrophins nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and neurotrophin-3 (NT-3) were evaluated. The proteins were continuously released by synthetic nerve guidance channels bridging a 4-mm gap in the transected dorsal root. Four weeks after lesion, the regenerated nerve cables were analyzed for the presence of myelinated and unmyelinated axons. While BDNF showed a limited effect on axonal regeneration (863 +/- 39 axons/regenerated nerve, n = 6), NGF (1843 +/- 482) and NT-3 (1495 +/- 449) powerfully promoted regeneration of myelinated axons compared to channels releasing the control protein bovine serum albumin (293 +/- 39). In addition, NGF, but not BDNF nor NT-3, had a potent effect on the regeneration of unmyelinated axons (NGF, 55 +/- 1.4; BDNF, 4 +/- 0.3; NT-3, 4.7 +/- 0.3 axons/100 microm(2); n = 6). The present study suggests that synthetic nerve guidance channels slowly and continuously releasing the neurotrophins NGF and NT-3 can overcome the limited regeneration of transected dorsal root.
Mots-clé
Animals Axons/physiology/ultrastructure Brain-Derived Neurotrophic Factor/administration & dosage/pharmacology Chick Embryo *Denervation Drug Delivery Systems Ganglia, Spinal/*drug effects/*physiopathology/ultrastructure Male Microscopy, Electron Nerve Fibers/physiology/ultrastructure Nerve Growth Factor/*administration & dosage/pharmacology Nerve Regeneration/*drug effects Neurotrophin 3/*administration & dosage/pharmacology Rats Rats, Wistar
Pubmed
Web of science
Création de la notice
06/02/2008 10:02
Dernière modification de la notice
20/08/2019 12:50
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