The correlation between microscopical examination and erythrocyte band 3 (AE1) gene deletion in South-east Asian ovalocytosis

Détails

ID Serval
serval:BIB_19A89F3F97F3
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
The correlation between microscopical examination and erythrocyte band 3 (AE1) gene deletion in South-east Asian ovalocytosis
Périodique
Transactions of the Royal Society of Tropical Medicine and Hygiène
Auteur(s)
Mgone  C. S., Genton  B., Peter  W., Paniu  M. M., Alpers  M. P.
ISSN
0035-9203 (Print)
Statut éditorial
Publié
Date de publication
06/1998
Volume
92
Numéro
3
Pages
296-9
Notes
Comparative Study
Journal Article --- Old month value: May-Jun
Résumé
South-east Asian ovalocytosis status was determined by microscopical examination of peripheral blood samples collected from 137 individuals in Papua New Guinea. The examination was performed separately by 2 microscopists, one of whom was very experienced in examining peripheral blood films for the diagnosis of south-east Asian ovalycytosis and the other was recently trained. The samples were also analysed by polymerase chain reaction (PCR) to determine ovalocytosis status by demonstrating a 27 base pair deletion in erythrocyte band 3 protein of the affected individuals. The microscopists were unaware of each other's results and of those obtained by PCR. Generally, there was very good agreement between the results obtained by both microscopists and the PCR. Although there was considerable inter-observer variation in the final ovalocyte count between the 2 microscopists, this did not affect their ability to discriminate between ovalocytic and normocytic individuals. Taking the PCR results as the standard, for the first, more experienced observer, the most efficient ovalocyte count cut-off point was around 50%. At this ovalocyte count the sensitivity and specificity of microscopical examination were 93.6% and 92.2%, and the positive and negative predictive values 86.3% and 96.5%, respectively. The second microscopist generally underscored the ovalocyte counts and his most efficient cut-off point was 20%, with sensitivity and specificity of 85.1% and 93.3% and positive and negative predictive values of 87.0% and 92.3%, respectively.
Mots-clé
Anion Exchange Protein 1, Erythrocyte/*genetics Elliptocytosis, Hereditary/*diagnosis Gene Deletion Humans Observer Variation Papua New Guinea Polymerase Chain Reaction/methods Sensitivity and Specificity
Pubmed
Web of science
Création de la notice
28/01/2008 11:49
Dernière modification de la notice
20/08/2019 12:50
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