DNA Uptake upon T6SS-Dependent Prey Cell Lysis Induces SOS Response and Reduces Fitness of Acinetobacter baylyi.
Détails
ID Serval
serval:BIB_1984349295B5
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
DNA Uptake upon T6SS-Dependent Prey Cell Lysis Induces SOS Response and Reduces Fitness of Acinetobacter baylyi.
Périodique
Cell reports
ISSN
2211-1247 (Electronic)
Statut éditorial
Publié
Date de publication
05/11/2019
Peer-reviewed
Oui
Volume
29
Numéro
6
Pages
1633-1644.e4
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Résumé
Certain Gram-negative bacteria use the type VI secretion system (T6SS) to kill and lyse competing bacteria. Here, we show that the T6SS-dependent lysis of prey cells by the naturally competent Acinetobacter baylyi results in the extensive filamentation of a subpopulation of A. baylyi cells. Filamentation is dependent on the release of DNA from the prey and its uptake by the competence system. The analysis of A. baylyi transcriptome and the response of transcriptional reporters suggest that the uptake of DNA results in the upregulation of the SOS response, which often leads to cell-division arrest. Long-term competition between competent and non-competent strains shows that the strain lacking the DNA uptake machinery outcompetes the parental strain only in the presence of the T6SS-dependent lysis of prey cells. Our data suggest that the cost of the induced SOS response may drive the selection of tight regulation or the loss of DNA uptake in bacteria capable of lysing their competitors.
Mots-clé
Acinetobacter/cytology, Acinetobacter/genetics, Acinetobacter/metabolism, Biological Transport, Cell Division, DNA, Bacterial/metabolism, Escherichia coli, Gene Expression Regulation, Bacterial/genetics, Microbial Interactions/genetics, Microbial Interactions/physiology, Promoter Regions, Genetic, Rec A Recombinases/genetics, Rec A Recombinases/metabolism, SOS Response, Genetics, Type VI Secretion Systems/metabolism, Acinetobacter, RecA, SOS response, cell division arrest, natural competence, type VI secretion
Pubmed
Web of science
Open Access
Oui
Création de la notice
22/07/2024 16:10
Dernière modification de la notice
27/07/2024 7:01