Organization and expression of the poxvirus genome.

Détails

ID Serval
serval:BIB_19456AF91B37
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Titre
Organization and expression of the poxvirus genome.
Périodique
Experientia
Auteur⸱e⸱s
Wittek R.
ISSN
0014-4754[print], 0014-4754[linking]
Statut éditorial
Publié
Date de publication
03/1982
Volume
38
Numéro
3
Pages
285-297
Langue
anglais
Notes
Publication types: Journal Article ; Review
Publication Status: ppublish
Résumé
Poxviruses comprise a large group of very complex animal DNA viruses which replicate in the cytoplasm of infected cells. Vaccinia virus, the most studied poxvirus, has a linear, double stranded DNA genome with an approximate molecular weight of 120 x 10(6) (180 kilobase pairs). The two strands of the DNA molecule are naturally cross-linked at both termini. In addition, the vaccinia virus genome contains very long inverted terminal repetitions of approximately 10 kilobase pairs which are further characterized by the presence of direct tandem repeats of a 70-base-pair sequence arranged in two blocks of 13 and 17 copies, respectively. A central region of the genome is highly conserved between different orthopoxviruses. In contrast, the ends are hypervariable and may contain extensive deletions and complex, symmetrical sequences rearrangements. Vaccinia virus gene expression is divided into two stages. Early in infection, RNA complementary to one half of one strand-equivalent of the genome is transcribed within subviral particles by the virion-associated RNA polymerase. Later in infection, after DNA replication, RNA complementary to one entire strand-equivalent is transcribed. RNA made late in infection is very heterogeneous in length and a large fraction of it contains self-complementary sequences. Late genes are clustered near the central region of the genome. Vaccinia virus mRNAs do not appear to be synthesized by a splicing mechanism.
Mots-clé
Base Sequence, DNA Replication, DNA Restriction Enzymes, DNA, Viral/genetics, Genes, Viral, Genetic Variation, Nucleic Acid Conformation, Poxviridae/genetics, RNA, Viral/genetics, Repetitive Sequences, Nucleic Acid, Species Specificity, Transcription, Genetic, Virus Replication
Pubmed
Web of science
Création de la notice
24/01/2008 10:43
Dernière modification de la notice
20/08/2019 12:50
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