The aliphatic amidase AmiE is involved in regulation of Pseudomonas aeruginosa virulence

Détails

ID Serval
serval:BIB_1923CE3245FD
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The aliphatic amidase AmiE is involved in regulation of Pseudomonas aeruginosa virulence
Périodique
Sci Rep
Auteur(s)
Clamens T., Rosay T., Crepin A., Grandjean T., Kentache T., Hardouin J., Bortolotti P., Neidig A., Mooij M., Hillion M., Vieillard J., Cosette P., Overhage J., O'Gara F., Bouffartigues E., Dufour A., Chevalier S., Guery B., Cornelis P., Feuilloley M. G., Lesouhaitier O.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Statut éditorial
Publié
Date de publication
2017
Volume
7
Pages
41178
Langue
anglais
Notes
Clamens, Thomas
Rosay, Thibaut
Crepin, Alexandre
Grandjean, Teddy
Kentache, Takfarinas
Hardouin, Julie
Bortolotti, Perrine
Neidig, Anke
Mooij, Marlies
Hillion, Melanie
Vieillard, Julien
Cosette, Pascal
Overhage, Joerg
O'Gara, Fergal
Bouffartigues, Emeline
Dufour, Alain
Chevalier, Sylvie
Guery, Benoit
Cornelis, Pierre
Feuilloley, Marc G J
Lesouhaitier, Olivier
eng
Research Support, Non-U.S. Gov't
England
Sci Rep. 2017 Jan 24;7:41178. doi: 10.1038/srep41178.
Résumé
We have previously shown that the eukaryotic C-type natriuretic peptide hormone (CNP) regulates Pseudomonas aeruginosa virulence and biofilm formation after binding on the AmiC sensor, triggering the amiE transcription. Herein, the involvement of the aliphatic amidase AmiE in P. aeruginosa virulence regulation has been investigated. The proteome analysis of an AmiE over-producing strain (AmiE(+)) revealed an expression change for 138 proteins, including some that are involved in motility, synthesis of quorum sensing compounds and virulence regulation. We observed that the AmiE(+) strain produced less biofilm compared to the wild type, and over-produced rhamnolipids. In the same line, AmiE is involved in P. aeruginosa motilities (swarming and twitching) and production of the quorum sensing molecules N-acyl homoserine lactones and Pseudomonas Quinolone Signal (PQS). We observed that AmiE overproduction reduced levels of HCN and pyocyanin causing a decreased virulence in different hosts (i.e. Dictyostelium discoideum and Caenorhabditis elegans). This phenotype was further confirmed in a mouse model of acute lung infection, in which AmiE overproduction resulted in an almost fully virulence decrease. Taken together, our data suggest that, in addition to its role in bacterial secondary metabolism, AmiE is involved in P. aeruginosa virulence regulation by modulating pilus synthesis and cell-to-cell communication.
Mots-clé
Amidohydrolases/*metabolism, Animals, Biofilms, Caenorhabditis elegans/microbiology, Dictyostelium/microbiology, Female, Lung/microbiology, Male, Mice, Inbred C57BL, Proteome, Pseudomonas Infections/*enzymology/microbiology, Pseudomonas aeruginosa/*enzymology/*pathogenicity, Quorum Sensing, Virulence, *Virulence Factors
Pubmed
Création de la notice
29/04/2021 10:59
Dernière modification de la notice
30/04/2021 6:38
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