A CDE/CHR-like element mediates repression of transcription of the mouse RB2 (p130) gene.

Détails

ID Serval
serval:BIB_191EA6A7CBC5
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
A CDE/CHR-like element mediates repression of transcription of the mouse RB2 (p130) gene.
Périodique
FEBS Letters
Auteur(s)
Fajas L., Le Cam L., Polanowska J., Fabbrizio E., Servant N., Philips A., Carnac G., Sardet C.
ISSN
0014-5793 (Print)
ISSN-L
0014-5793
Statut éditorial
Publié
Date de publication
2000
Volume
471
Numéro
1
Pages
29-33
Langue
anglais
Résumé
The bipartite repressor elements, termed cell cycle-dependent element (CDE)/cell cycle regulatory element (CCRE)-cell cycle homology region (CHR) control the growth-dependent transcription of the cyclin A, cdc25C, cdc2 genes. Here, we have identified a functional element displaying the signature of the CDE-CHR in the promoter of the mouse RB2 (p130) gene, encoding the retinoblastoma protein family (pRB)-related protein p130. This element locates close to the major transcription start site where it makes major groove contacts with proteins that can be detected in a cellular context using in vivo genomic footprinting techniques. Inactivation of either the CDE or CHR sequence strongly up-regulates the p130 promoter activity in exponentially growing cells, a situation where endogenous p130 gene expression is almost undetectable. Electrophoretic mobility shift assays suggest that two different protein complexes bind independently to the p130 CDE and CHR elements, and that the protein(s) bound to the CDE might be related to those bound on cyclin A and cdc2 promoters.
Mots-clé
Animals, Base Sequence, Cloning, Molecular, DNA, DNA-Binding Proteins/metabolism, Gene Expression Regulation, Genes, cdc, Humans, Mice, Molecular Sequence Data, Mutation, Phosphoproteins/genetics, Promoter Regions, Genetic, Proteins, Retinoblastoma-Like Protein p130, Sequence Homology, Nucleic Acid, Transcription, Genetic, Up-Regulation
Pubmed
Web of science
Open Access
Oui
Création de la notice
07/03/2013 16:09
Dernière modification de la notice
20/08/2019 12:49
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