Transcription of the TOL plasmid toluate catabolic pathway operon of Pseudomonas putida is determined by a pair of co-ordinately and positively regulated overlapping promoters.

Détails

ID Serval
serval:BIB_18B4634A4ABE
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Transcription of the TOL plasmid toluate catabolic pathway operon of Pseudomonas putida is determined by a pair of co-ordinately and positively regulated overlapping promoters.
Périodique
EMBO Journal
Auteur(s)
Mermod N., Lehrbach P.R., Reineke W., Timmis K.N.
ISSN
0261-4189[print], 0261-4189[linking]
Statut éditorial
Publié
Date de publication
11/1984
Volume
3
Numéro
11
Pages
2461-2466
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Expression of the meta-cleavage pathway operon of TOL plasmid pWW0 of Pseudomonas putida is positively regulated by the xylS gene product. We have sequenced the promoter region of this operon and localized the transcription initiation sites. Two overlapping promoters, designated Pm1 and Pm2, are responsible for the positively regulated expression of the meta-pathway operon. Mutants of P. putida were isolated that expressed the meta-cleavage pathway operon constitutively. Several plasmid-located mutations that led to constitutivity were characterized by sequencing and the transcription initiation sites on mutant plasmids localized. This resulted in the identification of newly created promoters whose functioning did not require the xylS product. Comparison of the promoter sequences obtained suggests a tentative consensus sequence for promoters of P. putida which is significantly different from that of E. coli.
Mots-clé
Base Sequence, DNA Restriction Enzymes, Endonucleases, Genes, Bacterial, Operon, Plasmids, Promoter Regions, Genetic, Pseudomonas/genetics, Single-Strand Specific DNA and RNA Endonucleases, Transcription, Genetic
Pubmed
Web of science
Création de la notice
24/01/2008 10:41
Dernière modification de la notice
20/08/2019 12:49
Données d'usage