TCR dynamics on the surface of living T cells.

Détails

ID Serval
serval:BIB_18934
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
TCR dynamics on the surface of living T cells.
Périodique
International Immunology
Auteur(s)
Favier B., Burroughs N.J., Wedderburn L., Valitutti S.
ISSN
0953-8178
Statut éditorial
Publié
Date de publication
2001
Volume
13
Numéro
12
Pages
1525-1532
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Résumé
T lymphocyte activation by specific antigen requires prolonged TCR occupancy and sustained signaling. This is accomplished by the formation of a specialized signaling domain, the immunological synapse, at the T cell-antigen-presenting cell contact site. Surface receptors and signaling components are progressively recruited into this domain where they are organized in defined three-dimensional structures. To better understand how TCR are supplied to the signaling domain during the activation process, we measured (using confocal microscopy and photo-bleaching recovery techniques) lateral mobility of GFP-tagged TCR on living Jurkat cell surface. We show that: (i) surface-expressed TCR exhibit an intrinsic, actin cytoskeleton-independent, lateral mobility which allows them to passively diffuse over the entire T cell surface within approximately 60 min and (ii) non-stimulated TCR rapidly enter the signaling domain. Our results indicate that TCR lateral mobility per se is sufficient to ensure TCR supply to the immunological synapse in the course of sustained T cell activation.
Mots-clé
Cell Membrane/genetics, Cell Membrane/immunology, Diffusion, Green Fluorescent Proteins, Humans, Jurkat Cells, Luminescent Proteins/genetics, Luminescent Proteins/metabolism, Microscopy, Confocal/methods, Protein Structure, Tertiary, Receptor-CD3 Complex, Antigen, T-Cell/biosynthesis, Receptor-CD3 Complex, Antigen, T-Cell/genetics, Signal Transduction/immunology, T-Lymphocytes/immunology, T-Lymphocytes/metabolism, Transfection
Pubmed
Web of science
Création de la notice
19/11/2007 12:13
Dernière modification de la notice
20/08/2019 12:49
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