Individuality of Ag-selected and preimmune TCR repertoires.

Détails

ID Serval
serval:BIB_18771
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Individuality of Ag-selected and preimmune TCR repertoires.
Périodique
Immunologic Research
Auteur(s)
Maryanski J.L., Attuil V., Hamrouni A., Mutin M., Rossi M., Aublin A., Bucher P.
ISSN
0257-277X
Statut éditorial
Publié
Date de publication
2001
Volume
23
Numéro
1
Pages
75-84
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Review Publication Status: ppublish
Résumé
We have combined flow cytometry and single-cell PCR to characterize the TCRBV repertoires selected by individual mice in a model CD8 response against a defined peptide/MHC complex (CW3 170-1 79/Kd). Ourresults established thatdifferent mice select individually distinct yet structurally similar CW3-specific repertoires. Repertoire selection appears to be flexible depending on the immunizing cell dose. Using a single-donor, matched-pair-recipient adoptive transfer strategy, we demonstrated that the CW3-specific TCR repertoires of normal mice are already distinct at the preimmune level. We combine our data with computer simulations to test models for the composition of an Ag-specific preimmune repertoire and its selection during an immune response.
Mots-clé
Animals, CD8-Positive T-Lymphocytes/immunology, Computer Simulation, Flow Cytometry, Gene Library, HLA-C Antigens/immunology, Humans, Mice, Polymerase Chain Reaction, Receptors, Antigen, T-Cell, alpha-beta/immunology
Pubmed
Web of science
Création de la notice
19/11/2007 10:40
Dernière modification de la notice
20/08/2019 13:48
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