L-tyrosine and nitric oxide synergize to prevent cytotoxic effects of superoxide.

Détails

ID Serval
serval:BIB_18500
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
L-tyrosine and nitric oxide synergize to prevent cytotoxic effects of superoxide.
Périodique
Toxicology
Auteur⸱e⸱s
Souici A.C., Fitzhugh A.L., Keefer L.K., Felley-Bosco E.
ISSN
0300-483X (Print)
ISSN-L
0300-483X
Statut éditorial
Publié
Date de publication
2001
Volume
165
Numéro
2-3
Pages
163-170
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S. Souici A.C. a repris son nom Peyter, AC
Résumé
We found previously that the nitric oxide donor DEA/NO enhanced lipid peroxidation, DNA fragmentation, and cytotoxicity in human bronchial epithelial cells (BEAS-2B) when they were cultured in LHC-8 medium containing the superoxide-generating system hypoxanthine/xanthine oxidase (HX/XO). We have now discovered that DEA/NO's prooxidant action can be reversed by raising the L-tyrosine concentration from 30 to 400 microM. DEA/NO also protected the cells when they were cultured in Dulbecco's Modified Eagle's Medium (DMEM), whose standard concentration of L-tyrosine is 400 microM. Similar trends were seen with the colon adenoma cell line CaCo-2. Since HPLC analysis of cell-free DMEM or LHC-8 containing 400 microM L-tyrosine, DEA/NO, and HX/XO revealed no evidence of L-tyrosine nitration, our data suggest the existence of an as-yet uncharacterized mechanism by which L-tyrosine can influence the biochemical and toxicological effects of reactive nitrogen species.
Mots-clé
Bronchi/cytology, Bronchi/drug effects, Caco-2 Cells/drug effects, Cell Line, Cell Survival/drug effects, DNA Damage, Drug Synergism, Humans, Hydrazines/pharmacology, Hydrazines/toxicity, Hypoxanthine/metabolism, Lipid Peroxidation/drug effects, Nitric Oxide/pharmacology, Nitric Oxide/toxicity, Nitric Oxide Donors/pharmacology, Nitric Oxide Donors/toxicity, Nitrogen Oxides, Reactive Oxygen Species/metabolism, Superoxides/antagonists & inhibitors, Superoxides/metabolism, Tyrosine/pharmacology, Xanthine Oxidase/metabolism
Pubmed
Web of science
Création de la notice
19/11/2007 12:12
Dernière modification de la notice
20/08/2019 12:48
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