A protective cocktail vaccine against murine cutaneous leishmaniasis with DNA encoding cysteine proteinases of Leishmania major.

Détails

ID Serval
serval:BIB_184E809001B1
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
A protective cocktail vaccine against murine cutaneous leishmaniasis with DNA encoding cysteine proteinases of Leishmania major.
Périodique
Vaccine
Auteur(s)
Rafati S., Salmanian A.H., Taheri T., Vafa M., Fasel N.
ISSN
0264-410X (Print)
ISSN-L
0264-410X
Statut éditorial
Publié
Date de publication
2001
Volume
19
Numéro
25-26
Pages
3369-3375
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
The protection elicited by the intramuscular injection of two plasmid DNAs encoding Leishmania major cysteine proteinase type I (CPb) and type II (CPa) was evaluated in a murine model of experimental cutaneous leishmaniasis. BALB/c mice were immunized either separately or with a cocktail of the two plasmids expressing CPa or CPb. It was only when the cpa and cpb genes were co-injected that long lasting protection against parasite challenge was achieved. Similar protection was also observed when animals were first immunized with cpa/cpb DNA followed by recombinant CPa/CPb boost. Analysis of the immune response showed that protected animals developed a specific Th1 immune response, which was associated with an increase of IFN-gamma production. This is the first report demonstrating that co-injection of two genes expressing different antigens induces a long lasting protective response, whereas the separate injection of cysteine proteases genes is not protective.
Mots-clé
Animals, Antibodies, Protozoan/biosynthesis, Antibodies, Protozoan/blood, Cysteine Endopeptidases/administration & dosage, Cysteine Endopeptidases/genetics, Female, Genes, Protozoan, Immunoglobulin G/biosynthesis, Immunoglobulin G/blood, Interferon-gamma/biosynthesis, Leishmania major/enzymology, Leishmania major/genetics, Leishmaniasis, Cutaneous/immunology, Leishmaniasis, Cutaneous/prevention & control, Mice, Mice, Inbred BALB C, Plasmids/genetics, Protozoan Vaccines/administration & dosage, Protozoan Vaccines/genetics, Vaccines, Combined/administration & dosage, Vaccines, DNA/administration & dosage, Vaccines, DNA/genetics, Vaccines, Synthetic/administration & dosage
Pubmed
Web of science
Création de la notice
24/01/2008 16:02
Dernière modification de la notice
20/08/2019 13:48
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