Differential effects of staphylococcal toxic shock syndrome toxin-1 on B cell apoptosis

Détails

ID Serval
serval:BIB_184D53B8D0AD
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Differential effects of staphylococcal toxic shock syndrome toxin-1 on B cell apoptosis
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur⸱e⸱s
Hofer  M. F., Newell  K., Duke  R. C., Schlievert  P. M., Freed  J. H., Leung  D. Y.
ISSN
0027-8424
Statut éditorial
Publié
Date de publication
05/1996
Peer-reviewed
Oui
Volume
93
Numéro
11
Pages
5425-30
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: May 28
Résumé
Superantigens, such as toxic shock syndrome toxin 1 (TSST-1), have been implicated in the pathogenesis of several autoimmune and allergic diseases associated with polyclonal B cell activation. In this report, we studied the in vitro effects of TSST-1 on B cell activation. We show herein that TSST-1 produced antagonistic effects on Ig synthesis by peripheral blood mononuclear cells (PBMC) from normal subjects, depending on the concentration used; Ig production was inhibited at 1000 pg/ml (P < 0.01) and enhanced at 1 and 0.01 pg/ml (P < 0.01) of toxin. Cultures of PBMC were then examined for morphologic features and DNA fragmentation characteristic for apoptosis. B cells exhibited a significantly higher (P < 0.01) incidence of apoptosis after stimulation with 1000 pg/ml of TSST-1 compared with 1 or 0.01 pg/ml of toxin or medium alone. Abundant expression of Fas, a cell surface protein that mediates apoptosis, was detected on B cells after stimulation with 1000 pg/ml of TSST-1 and was significantly higher on B cells undergoing apoptosis than on live cells (P = 0.01). Additionally, increased Fas expression and B cell death occurred at concentrations of TSST-1 inducing the production of high amounts of gamma interferon (IFN-gamma), and both events could be blocked by neutralizing anti-IFN-gamma antibody. These findings suggest that high concentrations of TSST-1 can induce IFN-gamma-dependent B cell apoptosis, whereas at low concentrations it stimulates Ig synthesis by PBMC from normal subjects. These findings support the concept that staphylococcal toxins have a role in B cell hyperactivity in autoimmunity and allergy.
Mots-clé
Animals Antibodies/pharmacology Antibody Formation/drug effects Apoptosis/*drug effects B-Lymphocytes/cytology/drug effects/*physiology *Bacterial Toxins Cells, Cultured Dose-Response Relationship, Drug Enterotoxins/*pharmacology Enzyme-Linked Immunosorbent Assay Flow Cytometry Humans Immunoglobulin G/pharmacology Interferon Type II/immunology/physiology Lymphocyte Activation/*drug effects Models, Immunological Rabbits Reference Values Staphylococcus aureus *Superantigens
Pubmed
Web of science
Open Access
Oui
Création de la notice
20/01/2008 16:23
Dernière modification de la notice
20/08/2019 13:48
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