A critical role for the T cell receptor alpha-chain connecting peptide domain in positive selection of CD1-independent NKT cells.

Détails

ID Serval
serval:BIB_18282
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
A critical role for the T cell receptor alpha-chain connecting peptide domain in positive selection of CD1-independent NKT cells.
Périodique
European Journal of Immunology
Auteur⸱e⸱s
Capone M., Troesch M., Eberl G., Hausmann B., Palmer E., MacDonald H.R.
ISSN
0014-2980
Statut éditorial
Publié
Date de publication
2001
Peer-reviewed
Oui
Volume
31
Numéro
6
Pages
1867-1875
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Résumé
Natural killer T (NKT) cells are a subset of mature alpha beta TCR(+) cells that co-express NK lineage markers. Whereas most NKT cells express a canonical Valpha14/Vbeta8.2 TCR and are selected by CD1d, a minority of NKT cells express a diverse TCR repertoire and develop independently of CD1d. Little is known about the selection requirements of CD1d-independent NKT cells. We show here that NKT cells develop in RAG-deficient mice expressing an MHC class II-restricted transgenic TCR (Valpha2/Vbeta8.1) but only under conditions that lead to negative selection of conventional T cells. Moreover development of NKT cells in these mice is absolutely dependent upon an intact TCR alpha-chain connecting peptide domain, which is required for positive selection of conventional T cells via recruitment of the ERK signaling pathway. Collectively our data demonstrate that NKT cells can develop as a result of high avidity TCR/MHC class II interactions and suggest that common signaling pathways are involved in the positive selection of CD1d-independent NKT cells and conventional T cells.
Mots-clé
Animals, Antigens, CD1/immunology, Binding Sites, CD8-Positive T-Lymphocytes/immunology, Hepatocytes, Histocompatibility Antigens Class II/immunology, Immunophenotyping, Killer Cells, Natural/immunology, Mice, Mice, Inbred C57BL, Mice, Knockout, Mice, Transgenic, Peptides/immunology, Receptors, Antigen, T-Cell, alpha-beta/genetics, Receptors, Antigen, T-Cell, alpha-beta/immunology
Pubmed
Web of science
Open Access
Oui
Création de la notice
19/11/2007 12:12
Dernière modification de la notice
20/08/2019 12:48
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