Transportin regulates nuclear import of CD44.

Détails

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Etat: Public
Version: Final published version
ID Serval
serval:BIB_17DB70E05151
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Transportin regulates nuclear import of CD44.
Périodique
Journal of Biological Chemistry
Auteur⸱e⸱s
Janiszewska M., De Vito C., Le Bitoux M.A., Fusco C., Stamenkovic I.
ISSN
1083-351X
0021-9258
ISSN-L
0021-9258
Statut éditorial
Publié
Date de publication
2010
Volume
285
Numéro
40
Pages
30548-30557
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
CD44 is a facultative cell surface proteoglycan that serves as the principal cell surface receptor for hyaluronan (HA). Studies have shown that in addition to participating in numerous signaling pathways, CD44 becomes internalized upon engagement by ligand and that a portion of its intracellular domain can translocate to the nucleus where it is believed to play a functional role in cell proliferation and survival. However, the mechanisms whereby fragments of CD44 enter the nucleus have not been elucidated. Here we show that CD44 interacts with two import receptors of the importin β superfamily, importin β itself and transportin. Inhibition of importin β-dependent transport failed to block CD44 accumulation in the nucleus. By contrast, inhibition of the transportin-dependent pathway abrogated CD44 import. Mutagenesis of the intracellular domain of CD44 revealed that the 20 membrane-proximal residues contain sequences required for transportin-mediated nuclear transport. Our observations provide evidence that CD44 interacts with importin family members and identify the transportin-dependent pathway as the mechanism whereby full-length CD44 enters the nucleus.
Pubmed
Web of science
Open Access
Oui
Création de la notice
26/08/2010 16:36
Dernière modification de la notice
20/08/2019 12:47
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