No measurable increase in thymidine glycol or 8-hydroxydeoxyguanosine in liver DNA of rats treated with nafenopin or choline-devoid low-methionine diet

Détails

ID Serval
serval:BIB_1778021AD574
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
No measurable increase in thymidine glycol or 8-hydroxydeoxyguanosine in liver DNA of rats treated with nafenopin or choline-devoid low-methionine diet
Périodique
Mutation Research
Auteur(s)
Hegi  M. E., Ulrich  D., Sagelsdorff  P., Richter  C., Lutz  W. K.
ISSN
0027-5107 (Print)
Statut éditorial
Publié
Date de publication
05/1990
Volume
238
Numéro
3
Pages
325-9
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May
Résumé
Male rats were treated for 2 months with 1000 ppm nafenopin in the diet or for 4 or 7 days with a choline-devoid low-methionine diet. DNA was isolated from the livers and analyzed for the presence of cis-thymidine glycol-3'-phosphate (cis-dTGp) by 32P-postlabeling and for the level of 8-hydroxy-deoxyguanosine (8-OH-dG) by electrochemical detection (ECD). In no DNA sample was the level of cis-dTGp above the limit of detection of 1 modified thymidine per 10(6) nucleotides. With 8-OH-dG, a background level of this modification of 20 8-OH-dG per 10(6) nucleosides was found in liver DNA of control rats, which was not affected by either treatment. It is postulated for thymidine glycol that a potential increase was below the limit of detection or was rapidly repaired in vivo and that the steady-state level of endogenous 8-hydroxydeoxyguanosine appears not to be influenced by the treatments chosen.
Mots-clé
Animals Choline Deficiency/*metabolism DNA/drug effects/*metabolism *DNA Damage Deoxyguanosine/analogs & derivatives/analysis Electrochemistry Liver/metabolism Male Methionine/deficiency/physiology Nafenopin/*pharmacology Propionates/*pharmacology Rats Rats, Inbred F344 Thymine/analogs & derivatives/analysis
Pubmed
Web of science
Création de la notice
25/01/2008 13:05
Dernière modification de la notice
20/08/2019 12:47
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