Bigh3 is upregulated in regenerating zebrafish fin.

Détails

ID Serval
serval:BIB_170C70B929E8
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Bigh3 is upregulated in regenerating zebrafish fin.
Périodique
Zebrafish
Auteur(s)
Page L., Polok B., Bustamante M., Schorderet D.F.
ISSN
1557-8542 (Electronic)
ISSN-L
1545-8547
Statut éditorial
Publié
Date de publication
2013
Peer-reviewed
Oui
Volume
10
Numéro
1
Pages
36-42
Langue
anglais
Notes
Publication types: Journal ArticlePublication Status: ppublish
Résumé
Zebrafish is a good model for studying regeneration because of the rapidity with which it occurs. Better understanding of this process may lead in the future to improvement of the regenerating capacity of humans. Signaling factors are the second largest category of genes, regulated during regeneration after the regulators of wound healing. Major developmental signaling pathways play a role in this multistep process, such as Bmp, Fgf, Notch, retinoic acid, Shh, and Wnt. In the present study, we focus on TGF-β-induced genes, bigh3 and bambia. Bigh3 encodes keratoepithelin, a protein first identified as an extracellular matrix protein reported to play a role in cell adhesion, as well as in cornea formation and osteogenesis. The expression of bigh3 in zebrafish fins has previously been reported. Here we demonstrate that tgf-b1 and tgf-b3 mRNA reacted with delay, first showing no regulation at 3âeuro0/00dpa, followed by upregulation at 4 and 5âeuro0/00dpa. Tgf-b1, tgf-2, and tgf-brII mRNA were back to normal levels at 10âeuro0/00dpa. Only tgf-b3 mRNA was still upregulated at that time. Bigh3 mRNA followed the upregulation of tgf-b1, while bambia mRNA behaved similarly to tgf-b2 mRNA. We show that upregulation of bigh3 and bambia mRNA correlated with the process of fin regeneration and regulation of TGF-b signaling, suggesting a new role for these proteins.
Mots-clé
Animal Fins/physiology, Animals, Extracellular Matrix Proteins/genetics, Extracellular Matrix Proteins/metabolism, Membrane Proteins/genetics, Membrane Proteins/metabolism, Osteogenesis, Regeneration, Signal Transduction, Transforming Growth Factor beta/genetics, Transforming Growth Factor beta/metabolism, Up-Regulation, Wound Healing, Zebrafish/genetics, Zebrafish/growth & development, Zebrafish Proteins/genetics, Zebrafish Proteins/metabolism
Pubmed
Web of science
Création de la notice
07/10/2013 11:27
Dernière modification de la notice
20/08/2019 13:46
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