Productive replication of adeno-associated virus can occur in human papillomavirus type 16 (HPV-16) episome-containing keratinocytes and is augmented by the HPV-16 E2 protein.

Détails

ID Serval
serval:BIB_17064
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Productive replication of adeno-associated virus can occur in human papillomavirus type 16 (HPV-16) episome-containing keratinocytes and is augmented by the HPV-16 E2 protein.
Périodique
Journal of Virology
Auteur(s)
Ogston P., Raj K., Beard P.
ISSN
0022-538X
Statut éditorial
Publié
Date de publication
2000
Volume
74
Numéro
8
Pages
3494-3504
Langue
anglais
Notes
Publication types: Journal Article
Résumé
We used a sensitive assay to test whether an adeno-associated virus (AAV) productive replication cycle can occur in immortalized human keratinocytes carrying episomal human papillomavirus type 16 (HPV-16) DNA. Following transfection with cloned AAV DNA, infectious AAV was produced, and the infectivity was blocked by anti-AAV antiserum. The HPV-16 E2 protein substantially increased the yield of AAV. Other HPV early proteins did not, in our experiments, show this ability. E2 has been shown to be able to affect p53 levels and to block cell cycle progression at mitosis. We tested the effect of changes in p53 expression on AAV replication and found that large differences in the level of p53 did not alter AAV DNA replication. In extension of this, we found that cellular help for AAV in response to stress was also independent of p53. To test if a mitotic block could trigger AAV DNA replication, we treated the cells with the mitotic inhibitor nocodazole. AAV DNA replication was stimulated by the presence of nocodazole in these and a number of other cell types tested. Yields of infectious virus, however, were not increased by this treatment. We conclude that the HPV-16 E2 protein stimulates AAV multiplication in these cells and propose that this occurs independently of the effects of E2 on p53 and cell cycle progression. Since the effect of E2 was not seen in keratinocytes lacking the HPV-16 episome, we suggest that E2 can help AAV by working in concert with other HPV-16 proteins.
Mots-clé
Animals, Antigens, Polyomavirus Transforming/immunology, Antigens, Polyomavirus Transforming/metabolism, COS Cells, Cell Line, Transformed, DNA-Binding Proteins, Dependovirus/genetics, Dependovirus/physiology, Humans, Keratinocytes/virology, Nocodazole/pharmacology, Nucleic Acid Amplification Techniques, Oncogene Proteins, Viral/genetics, Oncogene Proteins, Viral/metabolism, Papillomaviridae/genetics, Plasmids/genetics, Simian virus 40/immunology, Simian virus 40/metabolism, Transfection, Tumor Suppressor Protein p53/genetics, Tumor Suppressor Protein p53/metabolism, Virus Replication
Pubmed
Web of science
Open Access
Oui
Création de la notice
19/11/2007 13:10
Dernière modification de la notice
20/08/2019 13:46
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