The 18S ribosomal RNA m6A methyltransferase Mettl5 is required for normal walking behavior in Drosophila.
Détails
ID Serval
serval:BIB_1615D8B34DDC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
The 18S ribosomal RNA m6A methyltransferase Mettl5 is required for normal walking behavior in Drosophila.
Périodique
EMBO reports
ISSN
1469-3178 (Electronic)
ISSN-L
1469-221X
Statut éditorial
Publié
Date de publication
29/04/2020
Peer-reviewed
Oui
Pages
e49443
Langue
anglais
Notes
Publication types: Journal Article
Résumé
RNA modifications have recently emerged as an important layer of gene regulation. N6-methyladenosine (m <sup>6</sup> A) is the most prominent modification on eukaryotic messenger RNA and has also been found on noncoding RNA, including ribosomal and small nuclear RNA. Recently, several m <sup>6</sup> A methyltransferases were identified, uncovering the specificity of m <sup>6</sup> A deposition by structurally distinct enzymes. In order to discover additional m <sup>6</sup> A enzymes, we performed an RNAi screen to deplete annotated orthologs of human methyltransferase-like proteins (METTLs) in Drosophila cells and identified CG9666, the ortholog of human METTL5. We show that CG9666 is required for specific deposition of m <sup>6</sup> A on 18S ribosomal RNA via direct interaction with the Drosophila ortholog of human TRMT112, CG12975. Depletion of CG9666 yields a subsequent loss of the 18S rRNA m <sup>6</sup> A modification, which lies in the vicinity of the ribosome decoding center; however, this does not compromise rRNA maturation. Instead, a loss of CG9666-mediated m <sup>6</sup> A impacts fly behavior, providing an underlying molecular mechanism for the reported human phenotype in intellectual disability. Thus, our work expands the repertoire of m <sup>6</sup> A methyltransferases, demonstrates the specialization of these enzymes, and further addresses the significance of ribosomal RNA modifications in gene expression and animal behavior.
Mots-clé
Genetics, Biochemistry, Molecular Biology, Drosophila, Mettl5, RNA methyltransferase, behavior, m6A, ribosome
Pubmed
Web of science
Création de la notice
30/04/2020 23:48
Dernière modification de la notice
14/03/2024 7:09