Cell motion predicts human epidermal stemness

Détails

Ressource 1Télécharger: BIB_1601C33DDCF4.P001.pdf (2866.11 [Ko])
Etat: Public
Version: Final published version
ID Serval
serval:BIB_1601C33DDCF4
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Cell motion predicts human epidermal stemness
Périodique
Journal of Cell Biology
Auteur(s)
Nanba D., Toki F., Tate S., Imai M., Matsushita N., Shiraishi K., Sayama K., Toki H., Higashiyama S., Barrandon Y.
ISSN
0021-9525 (Print)
1540-8140 (Electronic)
ISSN-L
0021-9525
Statut éditorial
Publié
Date de publication
2015
Peer-reviewed
Oui
Volume
209
Numéro
2
Pages
305-315
Langue
anglais
Notes
Publication types: Research Articles ; research-article Identifiant PubMed Central: PMC4411274
Résumé
Image-based identification of cultured stem cells and noninvasive evaluation of their proliferative capacity advance cell therapy and stem cell research. Here we demonstrate that human keratinocyte stem cells can be identified in situ by analyzing cell motion during their cultivation. Modeling experiments suggested that the clonal type of cultured human clonogenic keratinocytes can be efficiently determined by analysis of early cell movement. Image analysis experiments demonstrated that keratinocyte stem cells indeed display a unique rotational movement that can be identified as early as the two-cell stage colony. We also demonstrate that α6 integrin is required for both rotational and collective cell motion. Our experiments provide, for the first time, strong evidence that cell motion and epidermal stemness are linked. We conclude that early identification of human keratinocyte stem cells by image analysis of cell movement is a valid parameter for quality control of cultured keratinocytes for transplantation.
Mots-clé
Cell Movement/physiology, Epidermis/cytology, Epidermis/metabolism, Integrin alpha6/genetics, Integrin alpha6/metabolism, Keratinocytes/cytology, Keratinocytes/metabolism, RNA, Messenger/genetics, Stem Cells/cytology, Stem Cells/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/07/2016 8:18
Dernière modification de la notice
20/08/2019 12:45
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