Antisense RNA directed against the 3' noncoding region prevents dormant mRNA activation in mouse oocytes.
Détails
ID Serval
serval:BIB_15EAAFA212DF
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Antisense RNA directed against the 3' noncoding region prevents dormant mRNA activation in mouse oocytes.
Périodique
Science
ISSN
0036-8075 (Print)
ISSN-L
0036-8075
Statut éditorial
Publié
Date de publication
05/08/1988
Peer-reviewed
Oui
Volume
241
Numéro
4866
Pages
680-684
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Publication Status: ppublish
Résumé
Primary mouse oocytes contain untranslated stable messenger RNA for tissue plasminogen activator (t-PA). During meiotic maturation, this maternal mRNA undergoes a 3'-polyadenylation, is translated, and is degraded. Injections of maturing oocytes with different antisense RNA's complementary to both coding and noncoding portions of t-PA mRNA all selectively blocked t-PA synthesis. RNA blot analysis of t-PA mRNA in injected, matured oocytes suggested a cleavage of the RNA.RNA hybrid region, yielding a stable 5' portion, and an unstable 3' portion. In primary oocytes, the 3' noncoding region was susceptible to cleavage, while the other portions of the mRNA were blocked from hybrid formation until maturation occurred. Injection of antisense RNA complementary to 103 nucleotides of its extreme 3' untranslated region was sufficient to prevent the polyadenylation, translational activation, and destabilization of t-PA mRNA. These results demonstrate a critical role for the 3' noncoding region of a dormant mRNA in its translational recruitment during meiotic maturation of mouse oocytes.
Mots-clé
Animals, Mice, Nucleic Acid Hybridization, Oocytes/metabolism, Poly A/metabolism, Protein Biosynthesis/drug effects, RNA/pharmacology, RNA, Antisense, RNA, Messenger/antagonists & inhibitors, RNA, Messenger/metabolism, Tissue Plasminogen Activator/genetics
Pubmed
Web of science
Création de la notice
24/10/2018 9:22
Dernière modification de la notice
25/05/2024 7:14