Gene order of the TOL catabolic plasmid upper pathway operon and oxidation of both toluene and benzyl alcohol by the xylA product.

Détails

ID Serval
serval:BIB_15B663716FD8
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Gene order of the TOL catabolic plasmid upper pathway operon and oxidation of both toluene and benzyl alcohol by the xylA product.
Périodique
Journal of Bacteriology
Auteur⸱e⸱s
Harayama S., Leppik R.A., Rekik M., Mermod N., Lehrbach P.R., Reineke W., Timmis K.N.
ISSN
0021-9193[print], 0021-9193[linking]
Statut éditorial
Publié
Date de publication
08/1986
Volume
167
Numéro
2
Pages
455-461
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
TOL plasmid pWW0 specifies enzymes for the oxidative catabolism of toluene and xylenes. The upper pathway converts the aromatic hydrocarbons to aromatic carboxylic acids via corresponding alcohols and aldehydes and involves three enzymes: xylene oxygenase, benzyl alcohol dehydrogenase, and benzaldehyde dehydrogenase. The synthesis of these enzymes is positively regulated by the product of xylR. Determination of upper pathway enzyme levels in bacteria carrying Tn5 insertion mutant derivatives of plasmid pWW0-161 has shown that the genes for upper pathway enzymes are organized in an operon with the following order: promoter-xylC (benzaldehyde dehydrogenase gene[s])-xylA (xylene oxygenase gene[s])-xylB (benzyl alcohol dehydrogenase gene). Subcloning of the upper pathway genes in a lambda pL promoter-containing vector and analysis of their expression in Escherichia coli K-12 confirmed this order. Two distinct enzymes were found to attack benzyl alcohol, namely, xylene oxygenase and benzyl alcohol dehydrogenase; and their catalytic activities were additive in the conversion of benzyl alcohol to benzaldehyde. The fact that benzyl alcohol is both a product and a substrate of xylene oxygenase indicates that this enzyme has a relaxed substrate specificity.
Mots-clé
Bacterial Proteins/genetics, Benzyl Alcohol, Benzyl Alcohols/metabolism, Benzyl Compounds/metabolism, Cloning, Molecular, DNA Transposable Elements, Escherichia coli/genetics, Genes, Bacterial, Mixed Function Oxygenases/genetics, Mixed Function Oxygenases/metabolism, Operon, Oxygenases/genetics, Oxygenases/metabolism, Phenotype, Plasmids, Pseudomonas/genetics, Substrate Specificity, Toluene/metabolism, Xylenes/metabolism
Pubmed
Web of science
Création de la notice
24/01/2008 10:41
Dernière modification de la notice
20/08/2019 12:44
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