Airway Microbiota Determines Innate Cell Inflammatory or Tissue Remodeling Profiles in Lung Transplantation.

Détails

ID Serval
serval:BIB_15B0E7AC9773
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Airway Microbiota Determines Innate Cell Inflammatory or Tissue Remodeling Profiles in Lung Transplantation.
Périodique
American journal of respiratory and critical care medicine
Auteur⸱e⸱s
Bernasconi E., Pattaroni C., Koutsokera A., Pison C., Kessler R., Benden C., Soccal P.M., Magnan A., Aubert J.D., Marsland B.J., Nicod L.P.
Collaborateur⸱rice⸱s
SysCLAD Consortium
ISSN
1535-4970 (Electronic)
ISSN-L
1073-449X
Statut éditorial
Publié
Date de publication
15/11/2016
Peer-reviewed
Oui
Volume
194
Numéro
10
Pages
1252-1263
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
In lung transplant recipients, long-term graft survival relies on the control of inflammation and tissue remodeling to maintain graft functionality and avoid chronic lung allograft dysfunction. Although advances in clinical practice have improved transplant success, the mechanisms by which the balance between inflammation and remodeling is maintained are largely unknown.
To assess whether host-microbe interactions in the transplanted lung determine the immunologic tone of the airways, and consequently could impact graft survival.
Microbiota DNA and host total RNA were isolated from 203 bronchoalveolar lavages obtained from 112 patients post-lung transplantation. Microbiota composition was determined using 16S ribosomal RNA analysis, and expression of a set of genes involved in prototypic macrophage functions was quantified using real-time quantitative polymerase chain reaction.
We show that the characteristics of the pulmonary microbiota aligned with distinct innate cell gene expression profiles. Although a nonpolarized activation was associated with bacterial communities consisting of a balance between proinflammatory (e.g., Staphylococcus and Pseudomonas) and low stimulatory (e.g., Prevotella and Streptococcus) bacteria, "inflammatory" and "remodeling" profiles were linked to bacterial dysbiosis. Mechanistic assays provided direct evidence that bacterial dysbiosis could lead to inflammatory or remodeling profiles in macrophages, whereas a balanced microbial community maintained homeostasis.
The crosstalk between bacterial communities and innate immune cells potentially determines the function of the transplanted lung offering novel pathways for intervention strategies.

Mots-clé
Adult, Airway Remodeling/physiology, Bronchoalveolar Lavage Fluid/microbiology, Female, Graft Survival/physiology, Humans, Inflammation/microbiology, Inflammation/physiopathology, Lung Transplantation, Male, Microbiota/physiology, Middle Aged, Respiratory System/microbiology, airway remodeling, macrophages, microbiome
Pubmed
Web of science
Création de la notice
14/06/2016 16:08
Dernière modification de la notice
20/08/2019 12:44
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