Cytokine-induced sleep: Neurons respond to TNF with production of chemokines and increased expression of Homer1a in vitro.

Détails

ID Serval
serval:BIB_15AA40436EC4
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Cytokine-induced sleep: Neurons respond to TNF with production of chemokines and increased expression of Homer1a in vitro.
Périodique
Brain, Behavior, and Immunity
Auteur(s)
Karrer M., Lopez M.A., Meier D., Mikhail C., Ogunshola O.O., Müller A.F., Strauss L., Tafti M., Fontana A.
ISSN
1090-2139 (Electronic)
ISSN-L
0889-1591
Statut éditorial
Publié
Date de publication
2015
Peer-reviewed
Oui
Volume
47
Numéro
6
Pages
186-192
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Résumé
Interactions of neurons with microglia may play a dominant role in sleep regulation. TNF may exert its somnogeneic effects by promoting attraction of microglia and their processes to the vicinity of dendrites and synapses. We found TNF to stimulate neurons (i) to produce CCL2, CCL7 and CXCL10, chemokines acting on mononuclear phagocytes and (ii) to stimulate the expression of the macrophage colony stimulating factor (M-CSF/Csf1), which leads to elongation of microglia processes. TNF may also act on neurons by affecting the expression of genes essential in sleep-wake behavior. The neuronal expression of Homer1a mRNA, increases during spontaneous and enforced periods of wakefulness. Mice with a deletion of Homer1a show a reduced wakefulness with increased non-rapid eye movement (NREM) sleep during the dark period. Recently the TNF-dependent increase of NREM sleep in the dark period of mice with CD40-induced immune activation was found to be associated with decreased expression of Homer1a. In the present study we investigated the effects of TNF and IL-1β on gene expression in cultures of the neuronal cell line HT22 and cortical neurons. TNF slightly increased the expression of Homer1a and IL-1β profoundly enhanced the expression of Early growth response 2 (Egr2). The data presented here indicate that the decreased expression of Homer1a, which was found in the dark period of mice with CD40-induced increase of NREM sleep is not due to inhibitory effects of TNF and IL-1β on the expression of Homer1a in neurons.
Mots-clé
Animals, Carrier Proteins/genetics, Carrier Proteins/metabolism, Cell Line, Cerebral Cortex/drug effects, Cerebral Cortex/metabolism, Chemokines/blood, Chemokines/metabolism, Early Growth Response Protein 2/genetics, Early Growth Response Protein 2/metabolism, Interleukin-1beta/pharmacology, Mice, Neurons/drug effects, Neurons/metabolism, Tumor Necrosis Factor-alpha/pharmacology
Pubmed
Web of science
Création de la notice
01/05/2014 17:57
Dernière modification de la notice
20/08/2019 12:44
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