Effect of intermittent hypoxic training on HIF gene expression in human skeletal muscle and leukocytes

Détails

ID Serval
serval:BIB_15A667CB85FA
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Effect of intermittent hypoxic training on HIF gene expression in human skeletal muscle and leukocytes
Périodique
European Journal of Applied Physiology
Auteur(s)
Mounier R., Pialoux V., Roels B., Thomas C., Millet G., Mercier J., Coudert J., Fellmann N., Clottes E.
ISSN
1439-6327[electronic]
Statut éditorial
Publié
Date de publication
11/2009
Volume
105
Numéro
4
Pages
515-524
Langue
anglais
Notes
Publication types: Journal Article
Résumé
Intermittent hypoxic exposure with exercise training is based on the assumption that brief exposure to hypoxia is sufficient to induce beneficial muscular adaptations mediated via hypoxia-inducible transcription factors (HIF). We previously demonstrated (Mounier et al. Med Sci Sports Exerc 38:1410-1417, 2006) that leukocytes respond to hypoxia with a marked inter-individual variability in HIF-1alpha mRNA. This study compared the effects of 3 weeks of intermittent hypoxic training on hif gene expression in both skeletal muscle and leukocytes. Male endurance athletes (n = 19) were divided into an Intermittent Hypoxic Exposure group (IHE) and a Normoxic Training group (NT) with each group following a similar 3-week exercise training program. After training, the amount of HIF-1alpha mRNA in muscle decreased only in IHE group (-24.7%, P < 0.05) whereas it remained unchanged in leukocytes in both groups. The levels of vEGF(121) and vEGF(165) mRNA in skeletal muscle increased significantly after training only in the NT group (+82.5%, P < 0.05 for vEGF(121); +41.2%, P < 0.05 for vEGF(165)). In leukocytes, only the IHE group showed a significant change in vEGF(165) (-28.2%, P < 0.05). The significant decrease in HIF-1alpha mRNA in skeletal muscle after hypoxic training suggests that transcriptional and post-transcriptional regulations of the hif-1alpha gene are different in muscle and leukocytes.
Mots-clé
Adult, Anoxia/genetics, Anoxia/metabolism, Gene Expression, Humans, Hypoxia-Inducible Factor 1, alpha Subunit/genetics, Leukocytes/metabolism, Male, Monocarboxylic Acid Transporters/genetics, Monocarboxylic Acid Transporters/metabolism, Muscle Proteins/genetics, Muscle Proteins/metabolism, Muscle, Skeletal/metabolism, Oxygen Consumption/genetics, RNA, Messenger/metabolism, Vascular Endothelial Growth Factor A/genetics, Vascular Endothelial Growth Factor A/metabolism
Pubmed
Création de la notice
20/11/2008 16:45
Dernière modification de la notice
20/08/2019 13:44
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