Caveolin-1 down-regulates inducible nitric oxide synthase via the proteasome pathway in human colon carcinoma cells.

Détails

ID Serval
serval:BIB_15814
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Caveolin-1 down-regulates inducible nitric oxide synthase via the proteasome pathway in human colon carcinoma cells.
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur⸱e⸱s
Felley-Bosco E., Bender F.C., Courjault-Gautier F., Bron C., Quest A.F.
ISSN
0027-8424 (Print)
ISSN-L
0027-8424
Statut éditorial
Publié
Date de publication
2000
Volume
97
Numéro
26
Pages
14334-14339
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
To investigate whether caveolin-1 (cav-1) may modulate inducible nitric oxide synthase (iNOS) function in intact cells, the human intestinal carcinoma cell lines HT29 and DLD1 that have low endogenous cav-1 levels were transfected with cav-1 cDNA. In nontransfected cells, iNOS mRNA and protein levels were increased by the addition of a mix of cytokines. Ectopic expression of cav-1 in both cell lines correlated with significantly decreased iNOS activity and protein levels. This effect was linked to a posttranscriptional mechanism involving enhanced iNOS protein degradation by the proteasome pathway, because (i) induction of iNOS mRNA by cytokines was not affected and (ii) iNOS protein levels increased in the presence of the proteasome inhibitors N-acetyl-Leu-Leu-Norleucinal and lactacystin. In addition, a small amount of iNOS was found to cofractionate with cav-1 in Triton X-100-insoluble membrane fractions where also iNOS degradation was apparent. As has been described for endothelial and neuronal NOS isoenzymes, direct binding between cav-1 and human iNOS was detected in vitro. Taken together, these results suggest that cav-1 promotes iNOS presence in detergent-insoluble membrane fractions and degradation there via the proteasome pathway.
Mots-clé
Caveolin 1, Caveolins/biosynthesis, Caveolins/genetics, Cell Fractionation, Colonic Neoplasms, Cysteine Endopeptidases/metabolism, Cytokines/metabolism, Cytokines/pharmacology, Detergents, Down-Regulation, Gene Expression, HT29 Cells, Humans, Multienzyme Complexes/metabolism, Nitric Oxide Synthase/biosynthesis, Nitric Oxide Synthase/genetics, Nitric Oxide Synthase Type II, Octoxynol, Protease Inhibitors/pharmacology, Proteasome Endopeptidase Complex, Signal Transduction, Solubility, Tumor Cells, Cultured
Pubmed
Web of science
Open Access
Oui
Création de la notice
19/11/2007 13:08
Dernière modification de la notice
20/08/2019 13:44
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