Deletion of the c-Ha-ras-1 allele in human skin cancers

Détails

ID Serval
serval:BIB_153E3798A855
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Deletion of the c-Ha-ras-1 allele in human skin cancers
Périodique
Molecular Carcinogenesis
Auteur(s)
Ananthaswamy  H. N., Applegate  L. A., Goldberg  L. H., Bales  E. S.
ISSN
0899-1987 (Print)
Statut éditorial
Publié
Date de publication
1989
Volume
2
Numéro
5
Pages
298-301
Notes
DA - 19900215
LA - eng
PT - Journal Article
PT - Research Support, Non-U.S. Gov't
PT - Research Support, U.S. Gov't, P.H.S
RN - 0 (DNA, Neoplasm)
SB - IM
Résumé
Previous studies have shown that c-Ha-ras-1 and other genes located on the short arm of chromosome 11 are frequently lost in a number of human tumors. We investigated whether similar losses of the c-Ha-ras-1 allele occurred in human squamous cell carcinomas (SCCs) and basal cell carcinomas (BCCs). DNAs were isolated from 35 pairs of skin tumors (25 BCCs and 10 SCCs) and matching normal skin from the same patients and analyzed for c-Ha-ras gene polymorphism by Southern blot hybridization. Sixteen BCC patients and 1 SCC patient were constitutionally heterozygous for the c-Ha-ras gene in their normal skin DNA. Of these 17 patients, five patients (four with BCC and one with SCC) (29%) showed loss of one of the c-Ha-ras alleles in their tumor DNA. One of the constitutionally heterozygous BCC patients exhibited deletion of the 6.6-kb c-Ha-ras allele and an extra copy of the 7.8-kb allele. In summary, loss of heterozygosity at the c-Ha-ras locus occurred frequently (29%) in the 17 human skin cancers studied. However, our finding that 90% of the patients with SCC, as opposed to 36% of the patients with BCC, had only one of the c-Ha-ras alleles in their normal skin tissue requires further study. Whether c-Ha-ras homozygosity has any bearing on genetic susceptibility to SCC remains to be established
Mots-clé
Alleles/Arm/Carcinoma,Basal Cell/Carcinoma,Squamous Cell/Chromosome Deletion/Dna/DNA,Neoplasm/Genes,ras/genetics/Heterozygote/Humans/immunology/Skin/Skin Neoplasms
Pubmed
Web of science
Création de la notice
18/02/2008 18:33
Dernière modification de la notice
20/08/2019 13:44
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