Exclusion of CD45 from the T-cell receptor signaling area in antigen-stimulated T lymphocytes.

Détails

ID Serval
serval:BIB_15029
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Exclusion of CD45 from the T-cell receptor signaling area in antigen-stimulated T lymphocytes.
Périodique
Current Biology
Auteur⸱e⸱s
Leupin O., Zaru R., Laroche T., Müller S., Valitutti S.
ISSN
0960-9822
Statut éditorial
Publié
Date de publication
2000
Volume
10
Numéro
5
Pages
277-280
Langue
anglais
Résumé
T lymphocytes are activated by the engagement of their antigen receptors (TCRs) with complexes of peptide and major histocompatibility complex (MHC) molecules displayed on the cell surface of antigen-presenting cells (APCs) [1]. An unresolved question of antigen recognition by T cells is how TCR triggering actually occurs at the cell-cell contact area. We visualized T-cell-APC contact sites using confocal microscopy and three-dimensional reconstruction of z-sections. We show the rapid formation of a specialized signaling domain at the T-cell-APC contact site that is characterized by a broad and sustained area of tyrosine phosphorylation. The T-lymphocyte cell-surface molecule CD2 is rapidly recruited into this signaling domain, whereas TCRs progressively percolate from the entire T-cell surface into the phosphorylation area. Remarkably, the highly expressed phosphatase CD45 is excluded from the signaling domain. Our results indicate that physiological TCR triggering at the T-cell-APC contact site is the result of a localized alteration in the balance between cellular kinases and phosphatases. We therefore provide experimental evidence to support current models of T-cell activation based on CD45 exclusion from the TCR signaling area [2] [3] [4].
Mots-clé
Antibodies, Monoclonal/metabolism, Antigen-Antibody Reactions, Antigen-Presenting Cells/metabolism, Antigens, CD45/metabolism, B-Lymphocytes/metabolism, Biological Markers, Humans, Microscopy, Confocal, Microscopy, Fluorescence, Phosphorylation, Receptors, Antigen, T-Cell/metabolism, Signal Transduction, T-Lymphocytes/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
19/11/2007 12:07
Dernière modification de la notice
20/08/2019 12:43
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