Nucleic acid-binding properties of the RRM-containing protein RDM1.

Détails

ID Serval
serval:BIB_14A65BCD8331
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Nucleic acid-binding properties of the RRM-containing protein RDM1.
Périodique
Biochemical and Biophysical Research Communications
Auteur(s)
Hamimes S., Bourgeon D., Stasiak A.Z., Stasiak A., Van Dyck E.
ISSN
0006-291X[print], 0006-291X[linking]
Statut éditorial
Publié
Date de publication
05/2006
Peer-reviewed
Oui
Volume
344
Numéro
1
Pages
87-94
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Résumé
RDM1 (RAD52 Motif 1) is a vertebrate protein involved in the cellular response to the anti-cancer drug cisplatin. In addition to an RNA recognition motif, RDM1 contains a small amino acid motif, named RD motif, which it shares with the recombination and repair protein, RAD52. RDM1 binds to single- and double-stranded DNA, and recognizes DNA distortions induced by cisplatin adducts in vitro. Here, we have performed an in-depth analysis of the nucleic acid-binding properties of RDM1 using gel-shift assays and electron microscopy. We show that RDM1 possesses acidic pH-dependent DNA-binding activity and that it binds RNA as well as DNA, and we present evidence from competition gel-shift experiments that RDM1 may be capable of discrimination between the two nucleic acids. Based on reported studies of RAD52, we have generated an RDM1 variant mutated in its RD motif. We find that the L119GF --> AAA mutation affects the mode of RDM1 binding to single-stranded DNA.
Mots-clé
Amino Acid Motifs/genetics, Amino Acid Sequence, Animals, Apoptosis, Cells, Cultured, Chickens, DNA, Single-Stranded/chemistry, DNA, Single-Stranded/ultrastructure, DNA-Binding Proteins/chemistry, DNA-Binding Proteins/genetics, Electrophoretic Mobility Shift Assay, Hydrogen-Ion Concentration, Molecular Sequence Data, Mutation, RNA/chemistry, RNA/ultrastructure, RNA-Binding Proteins/chemistry, RNA-Binding Proteins/genetics
Pubmed
Web of science
Création de la notice
24/01/2008 10:36
Dernière modification de la notice
20/08/2019 12:43
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