Identification of MAGI1 as a tumor-suppressor protein induced by cyclooxygenase-2 inhibitors in colorectal cancer cells.

Détails

Ressource 1Télécharger: BIB_148C3E6A199F.P001.pdf (827.79 [Ko])
Etat: Public
Version: Final published version
ID Serval
serval:BIB_148C3E6A199F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Identification of MAGI1 as a tumor-suppressor protein induced by cyclooxygenase-2 inhibitors in colorectal cancer cells.
Périodique
Oncogene
Auteur(s)
Zaric J., Joseph J.M., Tercier S., Sengstag T., Ponsonnet L., Delorenzi M., Rüegg C.
ISSN
1476-5594 (Electronic)
ISSN-L
0950-9232
Statut éditorial
Publié
Date de publication
2012
Volume
31
Numéro
1
Pages
48-59
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Cyclooxyganase-2 (COX-2), a rate-limiting enzyme in the prostaglandin synthesis pathway, is overexpressed in many cancers and contributes to cancer progression through tumor cell-autonomous and paracrine effects. Regular use of non-steroidal anti-inflammatory drugs or selective COX-2 inhibitors (COXIBs) reduces the risk of cancer development and progression, in particular of the colon. The COXIB celecoxib is approved for adjunct therapy in patients with Familial adenomatous polyposis at high risk for colorectal cancer (CRC) formation. Long-term use of COXIBs, however, is associated with potentially severe cardiovascular complications, which hampers their broader use as preventive anticancer agents. In an effort to better understand the tumor-suppressive mechanisms of COXIBs, we identified MAGUK with Inverted domain structure-1 (MAGI1), a scaffolding protein implicated in the stabilization of adherens junctions, as a gene upregulated by COXIB in CRC cells and acting as tumor suppressor. Overexpression of MAGI1 in CRC cell lines SW480 and HCT116 induced an epithelial-like morphology; stabilized E-cadherin and β-catenin localization at cell-cell junctions; enhanced actin stress fiber and focal adhesion formation; increased cell adhesion to matrix proteins and suppressed Wnt signaling, anchorage-independent growth, migration and invasion in vitro. Conversely, MAGI1 silencing decreased E-cadherin and β-catenin localization at cell-cell junctions; disrupted actin stress fiber and focal adhesion formation; and enhanced Wnt signaling, anchorage-independent growth, migration and invasion in vitro. MAGI1 overexpression suppressed SW480 and HCT116 subcutaneous primary tumor growth, attenuated primary tumor growth and spontaneous lung metastasis in an orthotopic model of CRC, and decreased the number and size of metastatic nodules in an experimental model of lung metastasis. Collectively, these results identify MAG1 as a COXIB-induced inhibitor of the Wnt/β-catenin signaling pathway, with tumor-suppressive and anti-metastatic activity in experimental colon cancer.
Mots-clé
Animals, Cell Adhesion, Cell Adhesion Molecules, Neuronal/physiology, Cell Line, Tumor, Cell Movement/drug effects, Colorectal Neoplasms/drug therapy, Colorectal Neoplasms/pathology, Cyclooxygenase 2 Inhibitors/pharmacology, Female, Humans, Lung Neoplasms/secondary, Mice, Neoplasm Invasiveness, Tumor Suppressor Proteins/physiology, Wnt Signaling Pathway, beta Catenin/physiology
Pubmed
Web of science
Open Access
Oui
Création de la notice
10/01/2013 9:34
Dernière modification de la notice
20/08/2019 13:43
Données d'usage