Efficient gene transfer and expression of biologically active glial cell line-derived neurotrophic factor in rat motoneurons transduced wit lentiviral vectors.

Détails

ID Serval
serval:BIB_14677
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Efficient gene transfer and expression of biologically active glial cell line-derived neurotrophic factor in rat motoneurons transduced wit lentiviral vectors.
Périodique
Journal of Neurochemistry
Auteur(s)
Cisterni C., Henderson C.E., Aebischer P., Pettmann B., Déglon N.
ISSN
0022-3042
Statut éditorial
Publié
Date de publication
2000
Peer-reviewed
Oui
Volume
74
Numéro
5
Pages
1820-1828
Langue
anglais
Résumé
Several studies have shown the ability of human immunodeficiency virus type 1 (HIV1)-based lentiviral vectors to infect nondividing brain and retinal neurons with high efficiency and long-term expression of the transduced gene. We show that purified embryonic motoneurons can be efficiently (>95%) transduced in culture using an HIV1-based lentiviral vector encoding LacZ. Expression of beta-galactosidase was observed for at least 9 days in these conditions. Furthermore, motoneurons transduced with a lentiviral vector expressing glial cell line-derived neurotrophic factor survived in the absence of additional trophic support, showing that the overexpressed protein was biologically active. Our results demonstrate the potential of lentiviral vectors in studying the biological effects of proteins expressed in motoneurons and in the development of future gene therapy for motoneuron diseases.
Mots-clé
Animals, Cell Survival/drug effects, Cells, Cultured, Embryo, Mammalian, Gene Expression, Gene Transfer Techniques, Genetic Vectors, Glial Cell Line-Derived Neurotrophic Factor, Lac Operon/genetics, Lentivirus/genetics, Motor Neurons/drug effects, Motor Neurons/metabolism, Nerve Growth Factors, Nerve Tissue Proteins/genetics, Nerve Tissue Proteins/metabolism, Rats
Pubmed
Web of science
Création de la notice
19/11/2007 13:07
Dernière modification de la notice
20/08/2019 13:43
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