Rapid detection of bacterial resistance to antibiotics using AFM cantilevers as nanomechanical sensors.

Détails

ID Serval
serval:BIB_140B510ED8B7
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Rapid detection of bacterial resistance to antibiotics using AFM cantilevers as nanomechanical sensors.
Périodique
Nature Nanotechnology
Auteur(s)
Longo G., Alonso-Sarduy L., Rio L.M., Bizzini A., Trampuz A., Notz J., Dietler G., Kasas S.
ISSN
1748-3395 (Electronic)
ISSN-L
1748-3387
Statut éditorial
Publié
Date de publication
2013
Peer-reviewed
Oui
Volume
8
Numéro
7
Pages
522-526
Langue
anglais
Résumé
The widespread misuse of drugs has increased the number of multiresistant bacteria, and this means that tools that can rapidly detect and characterize bacterial response to antibiotics are much needed in the management of infections. Various techniques, such as the resazurin-reduction assays, the mycobacterial growth indicator tube or polymerase chain reaction-based methods, have been used to investigate bacterial metabolism and its response to drugs. However, many are relatively expensive or unable to distinguish between living and dead bacteria. Here we show that the fluctuations of highly sensitive atomic force microscope cantilevers can be used to detect low concentrations of bacteria, characterize their metabolism and quantitatively screen (within minutes) their response to antibiotics. We applied this methodology to Escherichia coli and Staphylococcus aureus, showing that live bacteria produced larger cantilever fluctuations than bacteria exposed to antibiotics. Our preliminary experiments suggest that the fluctuation is associated with bacterial metabolism.
Pubmed
Web of science
Création de la notice
23/07/2013 12:43
Dernière modification de la notice
20/08/2019 12:42
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