Correlative light and electron microscopy using immunolabeled sections.

Détails

ID Serval
serval:BIB_13EC9D76BF73
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Correlative light and electron microscopy using immunolabeled sections.
Périodique
Methods In Molecular Biology
Auteur⸱e⸱s
Schwarz H., Humbel B.M.
ISSN
1940-6029 (Electronic)
ISSN-L
1064-3745
Statut éditorial
Publié
Date de publication
2014
Peer-reviewed
Oui
Volume
1117
Pages
559-592
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Résumé
In correlative microscopy, light microscopy provides the overview and orientation of the complex cells and tissue, while electron microscopy offers the detailed localization and correlation of subcellular structures. In this chapter we offer detailed high-quality electron microscopical preparation methods for optimum preservation of the cellular ultrastructure. From such preparations serial thin sections are collected and used for comparative histochemical, immunofluorescence, and immunogold staining.In light microscopy histological stains identify the orientation of the sample and immunofluorescence labeling facilitates to find the region of interest, namely, the labeled cells expressing the macromolecule under investigation. Sections, labeled with immunogold are analyzed by electron microscopy in order to identify the label within the cellular architecture at high resolution.
Mots-clé
Animals, Histocytological Preparation Techniques, Immunohistochemistry/methods, Microscopy/methods, Microscopy, Electron/methods, Rats, Zebrafish
Pubmed
Web of science
Création de la notice
27/08/2014 14:05
Dernière modification de la notice
20/08/2019 12:42
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