Genetic and Functional Characterization of an MCR-3-Like Enzyme-Producing Escherichia coli Isolate Recovered from Swine in Brazil.
Détails
ID Serval
serval:BIB_13B20AACD231
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Genetic and Functional Characterization of an MCR-3-Like Enzyme-Producing Escherichia coli Isolate Recovered from Swine in Brazil.
Périodique
Antimicrobial agents and chemotherapy
ISSN
1098-6596 (Electronic)
ISSN-L
0066-4804
Statut éditorial
Publié
Date de publication
07/2018
Peer-reviewed
Oui
Volume
62
Numéro
7
Pages
1
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Publication Status: epublish
Résumé
A collection of 126 pigs was screened for carriage of colistin-resistant Enterobacteriaceae in a farm in Minas Gerais, Brazil. Out of this collection, eight colistin-resistant Escherichia coli isolates were recovered, including one from Minas Gerais State producing a new MCR-3 variant (MCR-3.12). Analysis of the lipopolysaccharide revealed that MCR-3.12 had a function similar to that of MCR-1 and MCR-2 as a result of the addition of a phosphoethanolamine group to the lipid A moiety. Genetic analysis showed that the mcr-3.12 gene was carried by an IncA/C <sub>2</sub> plasmid and was embedded in an original genetic environment. This study reports the occurrence of the MCR-3-like determinant in South America and is the first to demonstrate the functionality of this group of enzymes as a phosphoethanolamine transferase.
Mots-clé
Animals, Anti-Bacterial Agents/pharmacology, Brazil, Colistin/pharmacology, Escherichia coli/drug effects, Escherichia coli/enzymology, Escherichia coli/genetics, Escherichia coli/isolation & purification, Escherichia coli Proteins/genetics, Escherichia coli Proteins/metabolism, Genetic Variation/genetics, Genome, Bacterial/genetics, Microbial Sensitivity Tests, Plasmids/genetics, Swine, Transferases (Other Substituted Phosphate Groups)/genetics, Transferases (Other Substituted Phosphate Groups)/metabolism, MCR-3, mcr, plasmid, polymyxins, swine
Pubmed
Web of science
Open Access
Oui
Création de la notice
03/05/2018 17:37
Dernière modification de la notice
24/09/2019 6:11