Genetic and Functional Characterization of an MCR-3-Like Enzyme-Producing Escherichia coli Isolate Recovered from Swine in Brazil.

Détails

ID Serval
serval:BIB_13B20AACD231
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Genetic and Functional Characterization of an MCR-3-Like Enzyme-Producing Escherichia coli Isolate Recovered from Swine in Brazil.
Périodique
Antimicrobial agents and chemotherapy
Auteur⸱e⸱s
Kieffer N., Nordmann P., Moreno A.M., Zanolli Moreno L., Chaby R., Breton A., Tissières P., Poirel L.
ISSN
1098-6596 (Electronic)
ISSN-L
0066-4804
Statut éditorial
Publié
Date de publication
07/2018
Peer-reviewed
Oui
Volume
62
Numéro
7
Pages
1
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Résumé
A collection of 126 pigs was screened for carriage of colistin-resistant Enterobacteriaceae in a farm in Minas Gerais, Brazil. Out of this collection, eight colistin-resistant Escherichia coli isolates were recovered, including one from Minas Gerais State producing a new MCR-3 variant (MCR-3.12). Analysis of the lipopolysaccharide revealed that MCR-3.12 had a function similar to that of MCR-1 and MCR-2 as a result of the addition of a phosphoethanolamine group to the lipid A moiety. Genetic analysis showed that the mcr-3.12 gene was carried by an IncA/C <sub>2</sub> plasmid and was embedded in an original genetic environment. This study reports the occurrence of the MCR-3-like determinant in South America and is the first to demonstrate the functionality of this group of enzymes as a phosphoethanolamine transferase.
Mots-clé
Animals, Anti-Bacterial Agents/pharmacology, Brazil, Colistin/pharmacology, Escherichia coli/drug effects, Escherichia coli/enzymology, Escherichia coli/genetics, Escherichia coli/isolation & purification, Escherichia coli Proteins/genetics, Escherichia coli Proteins/metabolism, Genetic Variation/genetics, Genome, Bacterial/genetics, Microbial Sensitivity Tests, Plasmids/genetics, Swine, Transferases (Other Substituted Phosphate Groups)/genetics, Transferases (Other Substituted Phosphate Groups)/metabolism, MCR-3, mcr, plasmid, polymyxins, swine
Pubmed
Web of science
Open Access
Oui
Création de la notice
03/05/2018 17:37
Dernière modification de la notice
24/09/2019 6:11
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