In vitro growth of human urinary tract smooth muscle cells on laminin and collagen type I-coated membranes under static and dynamic conditions.

Détails

ID Serval
serval:BIB_134467850F1B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
In vitro growth of human urinary tract smooth muscle cells on laminin and collagen type I-coated membranes under static and dynamic conditions.
Périodique
Tissue engineering
Auteur(s)
Hubschmid U., Leong-Morgenthaler P.M., Basset-Dardare A., Ruault S., Frey P.
ISSN
1076-3279
Statut éditorial
Publié
Date de publication
2005
Peer-reviewed
Oui
Volume
11
Numéro
1-2
Pages
161-71
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article - Publication Status: ppublish
Résumé
This study investigates in vitro growth of human urinary tract smooth muscle cells under static conditions and mechanical stimulation. The cells were cultured on collagen type I- and laminin-coated silicon membranes. Using a Flexcell device for mechanical stimulation, a cyclic strain of 0-20% was applied in a strain-stress-time model (stretch, 104 min relaxation, 15 s), imitating physiological bladder filling and voiding. Cell proliferation and alpha-actin, calponin, and caldesmon phenotype marker expression were analyzed. Nonstretched cells showed significant better growth on laminin during the first 8 days, thereafter becoming comparable to cells grown on collagen type I. Cyclic strain significantly reduced cell growth on both surfaces; however, better growth was observed on laminin. Neither the type of surface nor mechanical stimulation influenced the expression pattern of phenotype markers; alpha-actin was predominantly expressed. Coating with the extracellular matrix protein laminin improved in vitro growth of human urinary tract smooth muscle cells.
Mots-clé
Biological Markers, Cell Proliferation, Cells, Cultured, Collagen Type I, Female, Humans, Infant, Laminin, Male, Myocytes, Smooth Muscle, Stress, Mechanical, Urinary Bladder, Urinary Tract
Pubmed
Web of science
Création de la notice
28/02/2008 10:25
Dernière modification de la notice
20/08/2019 12:41
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