Determination of trace lithium in biological fluids using graphite furnace atomic absorption spectrophotometry: variability of urine matrices circumvented by cation exchange solid phase extraction.

Détails

ID Serval
serval:BIB_1322CA2919AC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Determination of trace lithium in biological fluids using graphite furnace atomic absorption spectrophotometry: variability of urine matrices circumvented by cation exchange solid phase extraction.
Périodique
Pharmaceutica acta Helvetiae
Auteur(s)
Magnin J.L., Decosterd L.A., Centeno C., Burnier M., Diezi J., Biollaz J.
ISSN
0031-6865 (Print)
ISSN-L
0031-6865
Statut éditorial
Publié
Date de publication
1996
Peer-reviewed
Oui
Volume
71
Numéro
4
Pages
237-246
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
A graphite furnace atomic absorption spectrometry method has been developed for the quantitative determination of submicromolar endogenous concentration of lithium in human plasma and urine using pyrolitically-coated graphite tubes in combination with ammonium nitrate matrix modification. This latter treatment could not completely abolish the interferences caused by the matrix, notably in urine samples. The variability of the urinary matrices required an additional standardization procedure by solid-phase extraction on strongly acidic cation exchange cartridges. Matrix-matched samples were used for the establishment of calibration curves with the addition-calibration method. Calibration curves were linear up to 0.72 mumol/l (1.0 > r2 > 0.99). The described method enables accurate measurements of trace-lithium in biological samples at concentrations down to 0.03 mumol/l with intra- and inter-day variabilities < 10%. The method was applied to the determination of trace-lithium levels in urine and plasma samples from healthy individuals enabling the calculation of its fractional excretion (FeLi) (median range 17.3%), a value which reflects the functional capacity of the kidney to reabsorb sodium and water at the proximal tubular portion of the nephron. This sensitive method can thus be used as an investigative and diagnostic tool in various renal pathophysiological conditions, in clinical research, and may also be applied to studies on the trace-lithium status of population in connection with psycho-affective disorders.
Mots-clé
Cations, Chromatography, Ion Exchange/methods, Humans, Lithium/blood, Lithium/urine, Microchemistry/methods, Reproducibility of Results, Spectrophotometry, Atomic
Pubmed
Création de la notice
21/01/2008 12:54
Dernière modification de la notice
10/09/2019 6:10
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