Ectopic expression of RET results in microphthalmia and tumors in the retinal pigment epithelium.

Détails

ID Serval
serval:BIB_12479
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Ectopic expression of RET results in microphthalmia and tumors in the retinal pigment epithelium.
Périodique
International journal of cancer. Journal international du cancer
Auteur(s)
Schmidt A., Tief K., Yavuzer U., Beermann F.
ISSN
0020-7136
Statut éditorial
Publié
Date de publication
1999
Volume
80
Numéro
4
Pages
600-605
Langue
anglais
Notes
Publication types: Journal Article
Résumé
The retinal pigment epithelium (RPE) is essential for eye development by interacting with the overlaying neuroepithelium. Regulatory sequences of the gene encoding for tyrosinase-related protein 1 (TRP-1), linked to the lacZ reporter gene, lead to strong and specific beta-galactosidase expression in the RPE. We asked how the oncogene ret would affect this epithelial cell type during mouse development. We used the TRP-1 promoter to express ret in the developing RPE, and obtained transgenic mouse lines, which showed mild to severe microphthalmia. During development, the RPE changed to a stratified epithelium with reduced or absent pigmentation from E10.5 onward. In addition, proliferation of RPE cells and tumor formation were observed from E12.5 onward. These early events prevent closure of choroid fissure and lead to microphthalmia and secondary malformations after birth. We conclude that ret transgene expression in the RPE prevents normal differentiation of this epithelial layer and induces proliferation and tumor formation. The appearance of the microphthalmic phenotype underlines the requirement of a normally developed RPE for eye development.
Mots-clé
Animals, Drosophila Proteins, Eye Neoplasms/embryology, Eye Neoplasms/genetics, Female, Gene Expression, Lac Operon, Male, Membrane Glycoproteins, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Transgenic, Microphthalmos/genetics, Oxidoreductases, Pigment Epithelium of Eye/embryology, Proteins/genetics, Proteins/metabolism, Proto-Oncogene Proteins/genetics, Proto-Oncogene Proteins/metabolism, Proto-Oncogene Proteins c-ret, Receptor Protein-Tyrosine Kinases/genetics, Receptor Protein-Tyrosine Kinases/metabolism, Recombinant Fusion Proteins/genetics, Recombinant Fusion Proteins/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
19/11/2007 12:03
Dernière modification de la notice
20/08/2019 12:40
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