Interleukin-2 (IL-2) regulates the accessibility of the IL-2-responsive enhancer in the IL-2 receptor alpha gene to transcription factors.

Détails

ID Serval
serval:BIB_12457
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Interleukin-2 (IL-2) regulates the accessibility of the IL-2-responsive enhancer in the IL-2 receptor alpha gene to transcription factors.
Périodique
Molecular and Cellular Biology
Auteur(s)
Rusterholz C., Henrioud P.C., Nabholz M.
ISSN
0270-7306
Statut éditorial
Publié
Date de publication
1999
Volume
19
Numéro
4
Pages
2681-2689
Langue
anglais
Notes
Publication types: Journal Article
Résumé
Interleukin-2 (IL-2) responsiveness of T lymphocytes is controlled through transcription of the IL-2 receptor (IL-2R) alpha subunit by antigen and by IL-2 itself. IL-2 induces IL-2Ralpha transcription via an IL-2-responsive enhancer (IL-2rE), whose activity depends on the cooperative binding of IL-2-induced STAT5 to two sites and of constitutively active Elf-1 to a third one. Here we describe the changes in IL-2rE chromatin that occur in normal T lymphocytes upon activation of IL-2Ralpha expression. In cells induced to transiently express IL-2Ralpha with concanavalin A (which mimics antigen), none of the IL-2rE sites is occupied despite the presence of Elf-1 and STAT1, which bind to the IL-2rE in vitro. The two STAT binding sites are occupied rapidly upon IL-2 stimulation, concomitantly with STAT5 activation. Occupation of the Elf-1 binding site is delayed, although Elf-1 concentration and binding activity are not modified by IL-2. Digestion of T-cell chromatin with DNase I and micrococcal nuclease shows that IL-2 induces the appearance of nuclease-hypersensitive sites flanking the IL-2rE. Thus IL-2, in addition to activating STAT5, appears to regulate IL-2Ralpha transcription by making IL-2Ralpha chromatin accessible to transcription factors.
Mots-clé
Animals, Concanavalin A/pharmacology, DNA Footprinting, DNA-Binding Proteins/metabolism, Enhancer Elements, Genetic, Gene Expression Regulation, Interleukin-2/pharmacology, Lymphocyte Activation, Mice, Mice, Inbred DBA, Milk Proteins, Nuclear Proteins, Nucleosomes/metabolism, Protein Binding, Receptors, Interleukin-2/biosynthesis, Receptors, Interleukin-2/genetics, Regulatory Sequences, Nucleic Acid, Response Elements, STAT1 Transcription Factor, STAT5 Transcription Factor, Spleen/cytology, T-Lymphocytes/cytology, T-Lymphocytes/metabolism, Trans-Activators/metabolism, Transcription Factors/metabolism
Pubmed
Web of science
Création de la notice
19/11/2007 12:03
Dernière modification de la notice
20/08/2019 12:40
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