Effect of thrombin inhibition on synovial inflammation in antigen induced arthritis

Détails

ID Serval
serval:BIB_11D3999F4701
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Effect of thrombin inhibition on synovial inflammation in antigen induced arthritis
Périodique
Annals of the Rheumatic Diseases
Auteur(s)
Varisco  P. A., Peclat  V., van Ness  K., Bischof-Delaloye  A., So  A., Busso  N.
ISSN
0003-4967 (Print)
Statut éditorial
Publié
Date de publication
10/2000
Volume
59
Numéro
10
Pages
781-7
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct
Résumé
OBJECTIVE: To determine the effect of the thrombin inhibitor, hirudin, on the pathogenesis of murine antigen induced arthritis (AIA). METHODS: AIA was induced by intra-articular injection of methylated bovine serum albumin in the knee joints of previously immunised mice. Hirudin (injected subcutaneously 3 x 200 microg/mouse/day) was given over 13 days, starting three days before arthritis onset, and its anticoagulant effect monitored by clotting times. Arthritis severity was evaluated by technetium-99m ((99m)Tc) uptake in the knee joints and by histological scoring. In addition, intra-articular fibrin deposition was examined by immunohistochemistry, and synovial cytokine mRNA expression measured by RNase protection. RESULTS: Joint inflammation, measured by (99m)Tc uptake, was significantly reduced in hirudin treated mice at days 7 and 10 after arthritis onset. Histologically, synovial thickness was markedly decreased in hirudin treated mice compared with untreated ones. By contrast, no difference in articular cartilage proteoglycan content was found between both groups. Intra-articular fibrin deposition and synovial interleukin 1beta mRNA levels, were slightly reduced ( approximately 20%) in arthritic joints from hirudin treated mice compared with untreated ones at day 10 of AIA. CONCLUSION: Hirudin reduces joint inflammation associated with AIA by fibrin-dependent and independent mechanisms.
Mots-clé
Animals Antithrombins/*therapeutic use Arthritis, Rheumatoid/*drug therapy/etiology/metabolism Cytokines/metabolism Drug Evaluation, Preclinical Fibrin/metabolism *Hirudin Therapy Interleukin-1/metabolism Mice Mice, Inbred C57BL Proteoglycans/metabolism Severity of Illness Index Synovitis/*drug therapy/etiology/metabolism Technetium/diagnostic use Treatment Outcome
Pubmed
Web of science
Création de la notice
25/01/2008 9:38
Dernière modification de la notice
20/08/2019 13:39
Données d'usage