Polymerase chain reaction (PCR) for detection of pathogenic microorganisms in bacteriological monitoring of dairy products.

Détails

ID Serval
serval:BIB_119A8F14BC7D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Polymerase chain reaction (PCR) for detection of pathogenic microorganisms in bacteriological monitoring of dairy products.
Périodique
Research in Microbiology
Auteur(s)
Allmann M., Höfelein C., Köppel E., Lüthy J., Meyer R., Niederhauser C., Wegmüller B., Candrian U.
ISSN
0923-2508 (Print)
ISSN-L
0923-2508
Statut éditorial
Publié
Date de publication
1995
Peer-reviewed
Oui
Volume
146
Numéro
1
Pages
85-97
Langue
anglais
Notes
Publication types: In Vitro ; Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Résumé
The presence of pathogenic bacteria poses a serious problem in sustaining the safety of dairy products. Microbiological routine controls of these products make use of selective culture techniques. To detect pathogenic species, isolated colonies are characterized by specific metabolic activities and by serotyping. We present an alternative biochemical approach that does not require culture of bacteria. The total bacterial populations of food samples were isolated by centrifugation and analysed by PCRs specific for pathogenic species. A total of 90 raw milk samples and dairy products made from raw milk were screened by this method for the presence of Listeria monocytogenes, Escherichia coli, enterotoxigenic E. coli, Campylobacter jejuni and C. coli. Detection rates were 12/90 (13%) for L. monocytogenes, 41/90 (46%) for E. coli, 18/90 (20%) for enterotoxigenic E. coli producing heat-labile toxin type I or heat-stable toxin type I, and 6/90 (7%) for C. jejuni or C. coli. Except for the use of different amplification primers, this approach is identical for any bacterial species to be detected. Direct PCR analysis of food samples offers rapid screening for the presence of specific bacteria and enables selection of critical samples prior to culture.
Mots-clé
Animals, Campylobacter coli/isolation & purification, Campylobacter jejuni/isolation & purification, Dairy Products, Electrophoresis, Agar Gel, Escherichia coli/isolation & purification, Food Microbiology, Listeria monocytogenes/isolation & purification, Milk, Polymerase Chain Reaction/methods
Pubmed
Web of science
Création de la notice
09/11/2014 16:45
Dernière modification de la notice
20/08/2019 13:39
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