Prospective isolation of functionally distinct radial glial subtypes--lineage and transcriptome analysis

Détails

ID Serval
serval:BIB_1198C6E2984A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Prospective isolation of functionally distinct radial glial subtypes--lineage and transcriptome analysis
Périodique
Mol Cell Neurosci
Auteur(s)
Pinto L., Mader M. T., Irmler M., Gentilini M., Santoni F., Drechsel D., Blum R., Stahl R., Bulfone A., Malatesta P., Beckers J., Gotz M.
ISSN
1095-9327 (Electronic)
ISSN-L
1044-7431
Statut éditorial
Publié
Date de publication
05/2008
Volume
38
Numéro
1
Pages
15-42
Langue
anglais
Notes
Pinto, Luisa
Mader, Michael T
Irmler, Martin
Gentilini, Marco
Santoni, Federico
Drechsel, Daniela
Blum, Robert
Stahl, Ronny
Bulfone, Alessandro
Malatesta, Paolo
Beckers, Johannes
Gotz, Magdalena
eng
Comparative Study
Research Support, Non-U.S. Gov't
Mol Cell Neurosci. 2008 May;38(1):15-42. doi: 10.1016/j.mcn.2008.01.012. Epub 2008 Feb 1.
Résumé
Since the discovery of radial glia as the source of neurons, their heterogeneity in regard to neurogenesis has been described by clonal and time-lapse analysis in vitro. However, the molecular determinants specifying neurogenic radial glia differently from radial glia that mostly self-renew remain ill-defined. Here, we isolated two radial glial subsets that co-exist at mid-neurogenesis in the developing cerebral cortex and their immediate progeny. While one subset generates neurons directly, the other is largely non-neurogenic but also gives rise to Tbr2-positive basal precursors, thereby contributing indirectly to neurogenesis. Isolation of these distinct radial glia subtypes allowed determining interesting differences in their transcriptome. These transcriptomes were also strikingly different from the transcriptome of radial glia isolated at the end of neurogenesis. This analysis therefore identifies, for the first time, the lineage origin of basal progenitors and the molecular differences of this lineage in comparison to directly neurogenic and gliogenic radial glia.
Mots-clé
Animals, Cell Lineage/*genetics, Cell Separation, Cells, Cultured, Cerebral Cortex/cytology/embryology/physiology, Gene Expression Profiling/*methods, Mice, Mice, Inbred C57BL, Mice, Transgenic, Neuroglia/*classification/cytology/*physiology, Rats, Rats, Wistar
Pubmed
Création de la notice
20/05/2019 13:52
Dernière modification de la notice
25/02/2020 7:26
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