Identification of receptor ligands by screening phage-display peptide libraries ex vivo on microdissected kidney tubules

Détails

ID Serval
serval:BIB_1169717A8FC9
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Identification of receptor ligands by screening phage-display peptide libraries ex vivo on microdissected kidney tubules
Périodique
Journal of the American Society of Nephrology
Auteur(s)
Odermatt  A., Audige  A., Frick  C., Vogt  B., Frey  B. M., Frey  F. J., Mazzucchelli  L.
ISSN
1046-6673 (Print)
Statut éditorial
Publié
Date de publication
02/2001
Volume
12
Numéro
2
Pages
308-16
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Feb
Résumé
A novel method to identify receptor ligands for defined renal tubular segments has been developed. Ex vivo screening of phage-display peptide libraries on isolated intact rat proximal convoluted tubules (PCT) and cortical collecting ducts (CCD) allowed the direct access of phage to the basolateral surface of tubular epithelial cells. Two distinct peptide motifs were selected for CCD and PCT, indicating differential expression of some membrane receptors on the basolateral surface of defined kidney tubule segments. Using the linear peptide motif ELRGD(R/M)AX(W/L), recovered from freshly isolated rat CCD, mediated 16-fold selectivity of phage binding to CCD compared with PCT. Binding to CCD was 39-fold higher than that of a random control phage. Binding and subsequent internalization of phage, most likely by an integrin-mediated endocytosis pathway, was abolished by the addition of the corresponding synthetic peptide. Furthermore, the results demonstrate that presentation and flanking amino acids determine the specific binding properties of RGD ligands to their putative integrin receptors. The results emphasize the need of a native cell system for the identification of renal epithelial cell surface ligands. Such ligands are of potential relevance for the analysis of interactions between extracellular matrix and kidney tubules or for the development of improved vectors for kidney-specific drug delivery or gene transfer.
Mots-clé
Animals Colonic Neoplasms/metabolism Dissection Kidney Tubules/*metabolism Ligands Male *Peptide Library Rats Rats, Sprague-Dawley Receptors, Cell Surface/*metabolism Receptors, Immunologic/metabolism Receptors, Peptide/metabolism
Pubmed
Web of science
Création de la notice
25/01/2008 14:03
Dernière modification de la notice
20/08/2019 13:39
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