PIDD orchestrates translesion DNA synthesis in response to UV irradiation.

Détails

ID Serval
serval:BIB_116367F1BB36
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
PIDD orchestrates translesion DNA synthesis in response to UV irradiation.
Périodique
Cell Death and Differentiation
Auteur⸱e⸱s
Logette E., Schuepbach-Mallepell S., Eckert M.J., Leo X.H., Jaccard B., Manzl C., Tardivel A., Villunger A., Quadroni M., Gaide O., Tschopp J.
ISSN
1476-5403 (Electronic)
ISSN-L
1350-9047
Statut éditorial
Publié
Date de publication
2011
Volume
18
Numéro
6
Pages
1036-1045
Langue
anglais
Résumé
PIDD has been implicated in survival and apoptotic pathways in response to DNA damage, and a role for PIDD was recently identified in non-homologous end-joining (NHEJ) repair induced by γ-irradiation. Here, we present an interaction of PIDD with PCNA, first identified in a proteomics screen. PCNA has essential functions in DNA replication and repair following UV irradiation. Translesion synthesis (TLS) is a process that prevents UV irradiation-induced replication blockage and is characterized by PCNA monoubiquitination and interaction with the TLS polymerase eta (polη). Both of these processes are inhibited by p21. We report that PIDD modulates p21-PCNA dissociation, and promotes PCNA monoubiquitination and interaction with polη in response to UV irradiation. Furthermore, PIDD deficiency leads to a defect in TLS that is associated, both in vitro and in vivo, with cellular sensitization to UV-induced apoptosis. Thus, PIDD performs key functions upon UV irradiation, including TLS, NHEJ, NF-κB activation and cell death.
Mots-clé
Apoptosis/genetics, Apoptosis/radiation effects, Carrier Proteins/genetics, Carrier Proteins/metabolism, Cell Line, DNA/biosynthesis, DNA/genetics, DNA Damage/genetics, DNA Damage/radiation effects, DNA Repair/genetics, DNA Repair/radiation effects, DNA Replication/genetics, DNA Replication/radiation effects, Gamma Rays, Humans, NF-kappa B/genetics, NF-kappa B/metabolism, Proliferating Cell Nuclear Antigen/genetics, Proliferating Cell Nuclear Antigen/metabolism, Ubiquitination/genetics, Ubiquitination/radiation effects, Ultraviolet Rays
Pubmed
Web of science
Open Access
Oui
Création de la notice
02/09/2011 9:59
Dernière modification de la notice
20/08/2019 13:39
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